Search results

From 2008.igem.org

Create the page "Standard Media" on this wiki!

  • Team:Heidelberg/Notebook/Killing II/10thweek
    ...CR-Screen: Colonies 1, 2, 3, 4, 7 & 8 positive; Inoculation of 5 ml LB-Amp media with this colonies for miniprep, 37 °C -> ON *Miniprep of pSB1A2-Receiver-ColE9lys (BioBrick Standard): colonies 1, 2, 3, 4, 7 & 8, eluted in 35 µl H<sub>2</sub>O
    43 KB (6,079 words) - 21:44, 28 October 2008
  • Team:Caltech/Project/Vitamins
    ...py via the addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG)to the media. This will allow us to test overexpression of each plasmid separately. In a ...haracterized using known quantities of folic acid in assay media. Once the standard curve has been established, then experimental growth levels, as quantified
    23 KB (3,375 words) - 18:48, 20 October 2008
  • Team:Heidelberg/Notebook/material
    ...gonucleotides were purchased from Invitrogen (Karlsruhe) and adjusted to a standard concentration of 100 <sup>pmol</sup>/<sub>µl</sub>. ===Bacteria Growth Media===
    69 KB (9,346 words) - 09:15, 20 August 2009
  • Team:Caltech/Protocols/Folate assay
    ...and sample tubes to light. I usually wrapped the buffers, assay media, and standard curve folic acid dilutions in aluminum foil. I also started covering the en ...ctobacilli Broth AOAC is recommended for ''L. rhamnosus'', so we used this media in place of the recommended Lactobacilli MRS broth.
    6 KB (840 words) - 00:48, 30 October 2008
  • Team:Heidelberg/Human Practice/Essay
    ...ut it. Furthermore we wanted to ask scientists about there opinion towards media contacts and the publication of their work for a broad audience. This knowl ...sk are positive: More scientists feel that they benefit from work with the media and popularizing their work than it would have been assumed.[[Team:Heidelbe
    41 KB (6,314 words) - 15:28, 29 October 2008
  • Team:Calgary Wetware/Protocols
    ...e foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> <li>Weigh 35g of LB-Agar powder mix per litre of media desired. One litre makes 40-50 plates</li>
    23 KB (3,681 words) - 23:18, 29 October 2008
  • GenProtocols
    #Grow 5mL culture of bacteria in LB media overnight, 37° C , shaking #Remove media and wash with 0.33 volumes (330μL) of 1M sorbitol
    17 KB (2,715 words) - 19:24, 26 October 2008
  • Jamboree/Project Abstract/Team Abstracts
    ...rupt. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick (http://gcat.davidson.edu/VizABrick/), ...h the following specifications: the promoters must conform to the BioBrick standard; they must be modular so they can be used multiply in devices; and they mus
    76 KB (11,186 words) - 15:27, 6 November 2008
  • Team:Cambridge/Improved GFP
    ...the only GFP variant currently listed and characterised in the Registry of Standard Biological Parts is the somewhat outdated GFP mut3b [3] . <br> ...anced versions of GFP – in standard BioBrick format - to the Registry of Standard Biological Parts would thus be expected to have a range of positive effects
    12 KB (1,879 words) - 03:48, 30 October 2008
  • Team:Hawaii/Make Competent E. Coli
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts].
    7 KB (1,158 words) - 23:54, 30 May 2008
  • Team:Hawaii/Protocols/Competent Cells
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts].
    5 KB (802 words) - 04:22, 24 June 2008
  • Team:IIT Madras/Project
    ...is an essential part of the synthetic biologist's toolkit. The Registry of Standard Biological Parts contains a growing number of promoters whose expression ca ...ements. We are currently characterizing the library of promoters against a standard control, the unmodified Lutz-Bujard LacO promoter.
    7 KB (1,125 words) - 14:42, 27 October 2008
  • Team:Caltech/Project/Oxidative Burst
    The constructs shown in figure 5 were made using the standard assembly method. They were made to test the various regulatory elements and ... arrow indicates induction with AHL at time 0 hr. Error bars represent one standard deviation(n=3) and are too small to be visible on the negative control.]]
    16 KB (2,564 words) - 20:23, 25 October 2008
  • Team:Heidelberg/Project/Killing II
    ...sually are not secreted, whereas colicins of group A are released into the media.[[Team:Heidelberg/Project/Killing_II#References|[4]]], [[Team:Heidelberg/Pr ...ot elucidated so far. Clear is that most of the colicin is released in the media, but only in a late state of release small amounts of lysis proteins were d
    65 KB (9,654 words) - 17:16, 29 October 2008
  • Team:Purdue/Project
    ...lacZ'' has been used for blue/white screening of ''E. coli'' for decades. Standard ''E. coli'' naturally make the lacZ protein (beta-galactosidase, or beta-ga ...ect one. For this project, we are using the ''recA'' promoter, which is a standard promoter of the SOS pathway and is activated only after extreme DNA damage.
    12 KB (1,859 words) - 23:49, 29 October 2008
  • Team:Harvard/Dailybook/Week1/Chemical and Light
    None of the samples in the LB amp media grew. All the others grew and then were miniprepped and stored in glycerol 6/26: Amp cultures still did not grow in liquid media, neither at 1:1000 or a 1:2000 dilution of amp
    34 KB (5,237 words) - 18:54, 26 October 2008
  • Team:Harvard/Dailybook/Week4/Chemical and Light
    8 100μL PCR reactions set up (standard mix): DNA template is S1 P13, 40 cycles, annealing temp is 58°C, extension 8 100μL PCR reactions set up (standard colony mix) w/ WT Shewanella
    38 KB (5,639 words) - 19:03, 26 October 2008
  • Minnesota/23 September 2008
    ...d fragment was cleaned by ethanol precipitation. The size of the reference standard promoter is 35 bp. (was obtained from source plate 1002, well number 10F). ...late (to preserve the individual clones) and inoculated separately into LB media to do plasmid isolation.
    3 KB (487 words) - 20:11, 29 October 2008
  • Template:Team:UC Berkeley/Notebook/MJV notes
    ...we talked about biobricks and cloning. Today we talked about assembly, BBb standard, and documentation. We also discussed our project plan today. In our demos, ...not have cultures on Sunday. I grew up cultures again on Sunday in new Amp media and was able to miniprep and run PCRs on the Cre and BglII templates today.
    43 KB (7,199 words) - 21:00, 25 September 2008
  • Template:Team:UC Berkeley/Notebook/MT notes
    ...resting to ''AS''--last year at iGEM, SynBERC received many questions from media sources about the novel education process involved in training new syntheti ...narrowed view of global environmental issues which are ever present in the media now--humans have been "fiddling with life," he said, for thousands of years
    52 KB (8,577 words) - 02:44, 30 October 2008
  • Team:Bologna/Biosafety
    ...terns in the building. Work is generally conducted on open benchtops using standard practices. Usually, "contaminated" materials are left in open (but separate # the recommended Standard Microbiological Practices, Special Practices, and Safety Equipment for BSL3
    29 KB (4,412 words) - 02:19, 30 October 2008
  • Materials and Methods
    Yeast were transformed using standard procedures (LiOAc method). ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]).
    3 KB (508 words) - 23:50, 29 October 2008
  • Team:TUDelft/Protocols
    * Grow a 5ml overnight culture of cells in LB media. * In the morning, dilute this culture back into 25-50ml of fresh LB media in a 200ml conical flask. You should aim to dilute the overnight culture by
    19 KB (3,279 words) - 23:04, 29 October 2008
  • Team:Harvard/GenProtocols
    #Remove media and wash with 0.33 volumes (330μL) of 1M sorbitol #Grow 5mL culture of bacteria in LB media overnight, 37° C , shaking
    13 KB (2,102 words) - 05:38, 29 October 2008
  • Team:Heidelberg/Notebook/Killing II/11thweek
    ... -> 200 µl Referencepromotercells + 200 µl Receivercells + 600 µl TB media ... -> 200 µl Referencepromotercells + 40 µl Receivercells + 760 µl TB media
    36 KB (4,963 words) - 21:50, 28 October 2008
  • Team:iHKU/protocol
    ... were collected by centrifugation, <em>ca</em>. 900 μl of supernatant media was discarded, and then the resuspended cells were plated onto LB agar con A 50 mL culture of EColi. was grown in 2% LB media overnight (16 hours) in 37’C shaker. A fresh 50 mL LB culture was inocul
    63 KB (6,466 words) - 19:25, 29 October 2008
  • Team:KULeuven/Data/GFP
    ==== Media ==== The media used were Luria-Bertani (LB) medium or ABT minimal medium. LB medium was us
    11 KB (1,747 words) - 15:41, 19 October 2008
  • Team:University of Washington/Project
    ...ry that provided a PoPS output when these three conditions are met. Using standard BioBrick assembly methods, we placed the gene for LuxR under the control of ...design of projects like ours easier using information from the Registry of Standard Biological Parts (see SeToB tab).
    23 KB (3,437 words) - 04:40, 30 October 2008
  • Team:Bologna/Protocols
    ...istilled water-EOH 95%) into the spot that corresponds to the Biobrick™-standard part that you want. # Put 5 ml of LB media in a falcon tube.
    13 KB (2,058 words) - 13:45, 28 October 2008
  • Team:NTU-Singapore/Wetlab/Materials and Equipment
    ==Bacteria strains and media== ...ht growth of cells were Luria-Bertani broth (LB). Cells were diluted in M9 media to an OD600 of 1 before ready to be read under Fluorescence Microplate Read
    6 KB (869 words) - 05:47, 27 October 2008
  • User:University of Washington/26 June 2008
    - Yeast growth media YEPD was obtained. - Yeast transforming media 50% PEG, 10x LiAc, and 10x TE were obtained.
    2 KB (251 words) - 00:25, 1 July 2008
  • Team:Michigan/Notebook/TransformationProtocol
    == <font color=royalblue size=5> Transformation Standard Protocol </font> == 7. Add 900 &mu;L pre-warmed LB media & allow to recover on spinning wheel in incubator (37 degrees Celsius) for
    2 KB (239 words) - 03:06, 30 October 2008
  • Team:Cambridge/Signaling
    Bacillus strain 1A1 (derivative of standard strain 168) * deficient in tryptophan, have to add to media
    7 KB (1,055 words) - 14:52, 28 October 2008
  • Team:Cambridge/Signalling
    Bacillus strain 1A1 (derivative of standard strain 168) * deficient in tryptophan, have to add to media
    6 KB (848 words) - 04:26, 30 October 2008
  • Team:ETH Zurich/Project/Background
    ...ticellularity or a nucleus can live a happy life with even less genes. The standard laboratory bacterium Escherichia coli is happy with just 4,467 genes (5, 6) ...s biosynthetic pathways that are not needed for growth in standard glucose media and that therefore only add to the costs of protein synthesis without incre
    9 KB (1,351 words) - 09:58, 30 October 2008
  • Everything you ever wanted to know about AarI
    ...lves the biggest problem faced when trying to do multipart ligations using standard restriction enzymes, the self ligation of a part, blocking it's incorporati ...serts). While parts can be made with any 4 base overhang (end), we chose a standard set, termed A, B, C, and D. This allows parts to be traded between research
    6 KB (1,025 words) - 04:20, 29 October 2008
  • Team:Bologna/Notebook
    ...rk: week by week you can find all the procedures, links to the registry of standard parts and protocols. The chronological structure of this section, organized ... to test and set up the new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol]
    15 KB (2,315 words) - 01:13, 30 October 2008
  • Team:Rice University/RESULTS
    ...nd the consumption of ''p''-coumaric acid, which will only be added to the media for the 4CL::STS-integrated yeast; the 4CL::STS+TAL will produce resveratro ...b|700px|'''Figure C1. Example HPLC chromatogram of a ''trans''-resveratrol standard.''']]
    7 KB (962 words) - 05:22, 30 October 2008
  • Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops
    [[Media:10-4-08 digest of 10;11;13.jpg]] [[Media:10-04-08 PCR verification.jpg]]
    7 KB (1,012 words) - 01:06, 29 October 2008
  • Team:Hawaii/Project/Part B MaterialsMethodsResults
    ...pilA'' and ''slr2016'' secretion signal sequences were syntheized with the standard Biobrick sites. Oligonucleotide fragments of each were hybridized with its Following standard PCR protocol, [http://www.partsregistry.org/Part:BBa_E0040 GFP] was convert
    13 KB (1,867 words) - 03:04, 30 October 2008
  • /Project/Vitamins
    ...nating in a double stop (TAATAA) sequence, as regulated by the Registry of Standard Biological Parts. ...e'' given known quantities of folate present in the growth media. Once the standard curve has been established, then experimental growth levels, as quantified
    10 KB (1,460 words) - 17:32, 22 August 2008
  • Team:Bologna/Software
    ...h segmented bacteria the software computes the size in pixel, the mean and standard deviation of 
intensity florescence. The use of the software is easy and ...of pixels) and obtain informations about their area, fluorescence mean and standard deviation. Fluorescence is read from R channel for RFP, G for GFP and B for
    6 KB (826 words) - 11:10, 27 April 2016
  • Team:Heidelberg/Notebook/Killing I/Notebook/week8
    * preparation of in vitro packaging media === Cloning oriT in standard plasmid ===
    27 KB (3,801 words) - 11:47, 29 October 2008
  • Main Page
    ...oree? See the <a class="pink" href="https://2008.igem.org/Jamboree/Media">Media</a> page for pictures and videos. Post your own links to let us know where **[[Jamboree/Media | Media ]]<small>(pictures & fun videos!)</small>
    10 KB (1,324 words) - 20:30, 5 May 2016
  • Team:Illinois/Antibody GPCR Fusion
    ...of the FUS1-Fluo.Prot. fusion into the yeast genome, which seems to be the standard for this type of thing. *Preparation of Yeast Media [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/m
    21 KB (3,227 words) - 02:19, 24 October 2008
  • Team:Hawaii/Project/Part B
    ...A'' and ''slr2016'' secretion signal sequences will be syntheized with the standard Biobrick sites. Oligonucleotide fragments of each will be hybridized with i ...efficacy of the signal peptides in transporting GFP into the extracellular media can be measured using a spectrofluorometer.
    11 KB (1,558 words) - 02:25, 29 October 2008
  • Template:Team:UC Berkeley/Notebook/CC notes
    2. Check all transfers form yesterday (sca1,2,4-9,14) on their anti plate and media growth 3. all media growth (including 4 of fimH) need to incubate a little longer
    23 KB (3,886 words) - 00:03, 9 August 2008
  • Team:University of Chicago/Notebook/Norayucel
    7. Added 250microliters SOC media (prepared at 1:30)<br> ...0microliters for pGreen, 1mL for E0240 (scaled up to accommodate for extra media dilution)
    11 KB (1,571 words) - 16:42, 28 September 2008
  • Team:EPF-Lausanne/Notebook
    ===Media and buffers=== ..., animals or the environment, other than the ones which are encountered in standard laboratory work with ''E.coli''. Therefore, we estimate the risk to researc
    16 KB (2,322 words) - 23:27, 29 October 2008
  • Team:Heidelberg/Human Practice/Open Day
    ...translated the text in english, the original german version can be found [[Media:IGEM-Veranstaltung_Heidelberg.pdf|'''here''']]). ...en_Day_english.ppt.html here] (rapdishare download file) and as pdf-file [[Media:Open_Day_english.pdf| here]]. <br/>
    60 KB (9,576 words) - 15:58, 29 October 2008
  • Team:UCSF/Materials and Methods
    ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]). ...ere grown to early log phase (OD600=0.1-0.3) in complete synthetic dropout media and then treated with 1 μM α-factor (Zymo Research) to activate the pathw
    3 KB (499 words) - 03:27, 30 October 2008
  • Team:Alberta NINT/Project
    [[Media:NINT_PlasmidDesignAndConstruction.pdf|Plasmid Design and Construction]] ...ion in the absence of any input. The y-axis is shown in Miller units (the standard for such assays).
    21 KB (3,119 words) - 16:44, 30 March 2009
  • Team:Hawaii/Member Training
    ## Read [[Image:Idempotent Vector Design for Standard Assembly of Biobricks.pdf|Biobrick Technical Manual]], don't worry about un ## [http://igem.blip.tv/file/254379 Drew Endy: What is a Standard Biological Part] 2 minute 31 seconds
    2 KB (309 words) - 12:16, 31 May 2008
  • Team:UC Berkeley/Protocols
    # Plate using the sterilized glass-bead method or with standard spreaders. # Plate using the sterilized glass-bead method or with standard spreaders.
    17 KB (2,857 words) - 22:03, 27 October 2008
  • Jamboree/Compete
    ...mboree/Compete#Requests_for_variance">Submit any requests for non-BioBrick standard methods</a> : <span class="deadline">October 8</span></li> ...xpected to submit their parts to the [http://partsregistry.org Registry of Standard Biological Parts]. In addition to submitting your parts, you must also dec
    13 KB (1,920 words) - 06:14, 22 April 2016
  • Team:Waterloo/Notebook
    ...plication written in-house for administration of BioBricks. It understands standard assembly and can gather information from the Parts Registry. BioMortar will ...hat it can construct sequences for composite parts automatically. Although Standard Assembly makes it easy to ligate parts, it is fairly easy to reverse the en
    3 KB (456 words) - 04:05, 30 October 2008
  • SOC
    #Follow directions to make 1 liter of [[SOB]] media [[Category:Material]] [[Category:Standard Media]]
    546 B (73 words) - 02:24, 30 May 2008
  • Team:Waterloo/Project
    ...nucleases during uninduced conditions. Cultures will initially be grown on media containing IPTG. Expression of the unstable cI repressor (C0051) will be dr ...me degradation construct will be integrated into the genome via a proposed standard integration vector.
    11 KB (1,711 words) - 04:00, 30 October 2008
  • Team:Groningen/Notebook
    ...e [http://partsregistry.org/Assembly:Standard_assembly <font color=#dd0000>Standard Assembly from the parts registry</font color>] site. When we had to assembl '''Media'''
    4 KB (643 words) - 12:10, 16 September 2008
  • Alberta NINT/23 July 2008
    showed sufficient cell growth after two days at 37 C. Prepared media (LB+antibiotics+IPTG+glucose/arabinose) and inoculated 1 ml of media with 20 ul of cell cultures
    968 B (108 words) - 23:02, 11 October 2008
  • Team:Davidson-Missouri Western
    ...rts. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick, and other web-based tools to improve t i'''GEM 2008 powerpoint presentation''' [[Media:MWSU_Davidson_iGEM08.PPT]]
    4 KB (583 words) - 03:55, 30 October 2008
  • Team:University of Washington/Measurement Kit
    ... After 16 hours, each overnight culture was diluted 100 fold into fresh M9 media with kanamycin, and grown for 3 hours. After three hours, two 200uL samples
    4 KB (577 words) - 04:09, 30 October 2008
  • Team:Peking University/Project
    ...s yeast codon optimized kindly offered by Prof. Youri I Pavlov. A set of [[Media:Linkers.pdf|12 linkers]] were synthesized to test the ability to maintain t ...flanking the 3’ of the promoter. Other constructs were accomplished with standard protocols.
    18 KB (2,815 words) - 05:26, 30 October 2008
  • Team:Heidelberg/Notebook/Killing II/5thweek
    *Retransformation of ligation into TOP 10 -> standard protocoll *Preparation of M9 media
    27 KB (3,683 words) - 21:27, 28 October 2008
  • Brown: Team Resistance/14 July 2008
    *Lyse cells in minimal media instead of LB because the ion content of minimal media is controlled and known *use standard copper wire in the breadboard for the circuit and only use gold/platinum fo
    5 KB (771 words) - 18:58, 29 October 2008
  • Teachtheteachers
    <b>10:00 am -- Jason Kelly -- Standard Measurement Kit for BioBrick Promoters (PoPS-out devices)</b><br> ...xplains how to measure the PoPs of promoters and compare it to a reference standard.
    5 KB (778 words) - 20:34, 8 May 2008
  • Team:ETH Zurich/Project/Motivation
    ...ard E. coli, the cells with reduced genome grew slightly denser in certain media, tolerated a greater variety of plasmids and had higher electroporation eff
    6 KB (907 words) - 09:57, 30 October 2008
  • Team:Caltech/Protocols/Measuring H2O2
    ...(100uM, 33.3uM, 11.1 uM, 3.7 uM and 0.0 uM H2O2) made in the corresponding media. [[Image:H2O2 standards example.png|thumb|center|400px|An example of a typical standard curve generated by the assay. Error bars (1SD, n=3) are too small to be see
    1 KB (162 words) - 00:24, 30 October 2008
  • SOB
    [[Category:Material]] [[Category:Standard Media]]
    1 KB (171 words) - 02:13, 31 May 2008
  • Team:Illinois/Antibody RTK Fusion
    ...pplies needed will include the cholera B subunit, yeast culture media, and standard molecular biology reagents/kits.
    7 KB (1,026 words) - 04:36, 30 October 2008
  • User:University of Washington/30 June 2008
    - Spotted filter paper sections were removed for the fully-assembled standard reference promoter-RBS-GFP gene-backbone plasmid (I20260), and three differ - 250 uL of SOC media (heated to 37 degrees Celsius) was added to each of the TOP10 tubes, then i
    3 KB (456 words) - 22:59, 2 July 2008
  • User:University of Washington/17 July 2008
    ...d both by myself and Scott. PCR failed. Will attempt a control PCR using standard BioBrick primers. Putative error: dNTPs? - 1200 uL of yeast in liquid YEPD media was separated into two Eppendorf tubes. These were centrifuged and pelleted
    2 KB (376 words) - 23:19, 22 July 2008
  • Team:Freiburg/Project
    ...he construction of fusion proteins due to extended Biobrick 3.0 (Freiburg) standard. Cloning was done in <i>E. coli</i>. However, we test our receptors in huma ...Origami-yield and measurement of the molecule´s stability in cell culture media the later ones.<br>
    12 KB (1,733 words) - 08:24, 30 October 2008
  • Team:Illinois/Antibody GPCR Fusion Notebook
    ** Brought to standard concentration, 30uM *Add SOC Media (200uL)
    13 KB (1,969 words) - 02:55, 30 October 2008
  • Team:TUDelft/Ethics conclusions
    '''Full report: Click this [[Media:TUDelft_iGEM_Ethics_StevenF.pdf|link]]''' ... involved and hence the good products will not be added to the registry of standard biological parts.
    20 KB (3,152 words) - 19:44, 29 October 2008
  • Team:Chiba/Sender experiments
    #Inoculated them independently in liquid media. Incubated at 37°C 12h ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synth
    6 KB (641 words) - 12:14, 29 October 2008
  • Team:Brown/Notebook/Timeline
    ...d, our apparatus became more complex. We quickly made the transition from standard voltmeter to a Data Acquisition Card coupled with LabView Computer Software *We also experimented with LB Lennox, Luria, M9 Minimal Media, Distilled/Sterile Water, as well as Milli-Q water. Cells were pelleted an
    6 KB (917 words) - 17:35, 29 October 2008
  • Team:Heidelberg/Notebook/Killing I/Notebook/week12
    * the insert was ligated in the lambda backbone over night using standard ligation methods * colonies were picked and transferred in liquid media containing chloramphenicol
    36 KB (4,978 words) - 14:47, 29 October 2008
  • Team:Heidelberg/Human Practice/Phips the Phage/Technical Backround
    ...transformed bacteria to survive, if antibiotics are present in the culture media. All untransformed cells die due to the added antibiotics, because they do ...itcal introduction, the pupils carry out a plasmid minipreparation using a standard kit.
    51 KB (8,118 words) - 16:56, 29 October 2008
  • Team:Imperial College/B.subtilis
    ... subtilis</i> chassis page</b></a></html> on the '''Registry of Biological Standard Parts'''. We characterised the growth rate of ''B. subtilis'' in LB media at 37<sup>o</sup>C. This curve provides useful information when inducing pr
    3 KB (492 words) - 03:41, 30 October 2008
  • Team:Slovenia/Notebook/Methods
    ...r the overexpression of flagellar proteins.&nbsp;Cultures were grown in LB media (Luria-Bertani) supplemented with ampicillin&nbsp;at 37&deg;C with shaking ...dividually numbered by ear-notching, regularly examined, weighed and fed a standard rodent chow (Altromin 1324, Lage, Germany). Food was available <i>ad libitu
    48 KB (6,976 words) - 04:42, 30 October 2008
  • Team:Davidson-Missouri Western/Viz-A-Brick: A New Way to Visualize the Registry
    As the Registry of Standard Biological Parts expands, it becomes increasingly important that users be a [[Media:Example.ogg]]
    3 KB (484 words) - 18:13, 29 October 2008
  • Team:Chiba/Project/Experiments:Crosstalk
    ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI,RhlI,LasI means fluorescence induced by AHLs synth #Inoculated them independently in liquid media. Incubated at 37°C 12h
    7 KB (579 words) - 12:43, 29 October 2008
  • Team:Cambridge/Voltage/Lab Work
    ...increase volume in preparation for experiments tomorrow on K+ tolerance in media. * Designed standard infusion primers for biobrick assembly. Abandoned due to unfeasibly large s
    21 KB (3,118 words) - 23:31, 29 October 2008
  • Team:Chiba/Project/Experiments:Sender Crosstalk
    #Inoculated them independently in liquid media. Incubated at 37&deg;C for 12hours. ...ts are averages from three replicate cultures with error bars representing standard deviations.Labeling:LuxI, RhlI, and LasI means fluorescence induced by AHLs
    7 KB (1,039 words) - 11:00, 30 October 2008
  • Team:Groningen/references.html
    ...ttp://openwetware.org/wiki/Griffitts:Common_media">Openwetware.org: Common Media</a></li> ...tp://partsregistry.org/Assembly:Standard_assembly">Partsregistry.org Help: Standard Assembly</a></li>
    7 KB (924 words) - 00:22, 30 October 2008

View (previous 500 | next 500) (20 | 50 | 100 | 250 | 500)