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  • Team:Heidelberg/Notebook/Killing II/10thweek
    ...CR-Screen: Colonies 1, 2, 3, 4, 7 & 8 positive; Inoculation of 5 ml LB-Amp media with this colonies for miniprep, 37 °C -> ON *Miniprep of pSB1A2-Receiver-ColE9lys (BioBrick Standard): colonies 1, 2, 3, 4, 7 & 8, eluted in 35 µl H<sub>2</sub>O
    43 KB (6,079 words) - 21:44, 28 October 2008
  • Team:Caltech/Project/Vitamins
    ...py via the addition of Isopropyl β-D-1-thiogalactopyranoside (IPTG)to the media. This will allow us to test overexpression of each plasmid separately. In a ...haracterized using known quantities of folic acid in assay media. Once the standard curve has been established, then experimental growth levels, as quantified
    23 KB (3,375 words) - 18:48, 20 October 2008
  • Team:Heidelberg/Notebook/material
    ...gonucleotides were purchased from Invitrogen (Karlsruhe) and adjusted to a standard concentration of 100 <sup>pmol</sup>/<sub>µl</sub>. ===Bacteria Growth Media===
    69 KB (9,346 words) - 09:15, 20 August 2009
  • Team:Caltech/Protocols/Folate assay
    ...and sample tubes to light. I usually wrapped the buffers, assay media, and standard curve folic acid dilutions in aluminum foil. I also started covering the en ...ctobacilli Broth AOAC is recommended for ''L. rhamnosus'', so we used this media in place of the recommended Lactobacilli MRS broth.
    6 KB (840 words) - 00:48, 30 October 2008
  • Team:Heidelberg/Human Practice/Essay
    ...ut it. Furthermore we wanted to ask scientists about there opinion towards media contacts and the publication of their work for a broad audience. This knowl ...sk are positive: More scientists feel that they benefit from work with the media and popularizing their work than it would have been assumed.[[Team:Heidelbe
    41 KB (6,314 words) - 15:28, 29 October 2008
  • Team:Calgary Wetware/Protocols
    ...e foil with a pipette tip into the well that corresponds to the Biobrick - standard part that you want</li> <li>Weigh 35g of LB-Agar powder mix per litre of media desired. One litre makes 40-50 plates</li>
    23 KB (3,681 words) - 23:18, 29 October 2008
  • GenProtocols
    #Grow 5mL culture of bacteria in LB media overnight, 37° C , shaking #Remove media and wash with 0.33 volumes (330μL) of 1M sorbitol
    17 KB (2,715 words) - 19:24, 26 October 2008
  • Jamboree/Project Abstract/Team Abstracts
    ...rupt. We also produced a graphical interface for exploring the Registry of Standard Biological Parts called Viz-A-Brick (http://gcat.davidson.edu/VizABrick/), ...h the following specifications: the promoters must conform to the BioBrick standard; they must be modular so they can be used multiply in devices; and they mus
    76 KB (11,186 words) - 15:27, 6 November 2008
  • Team:Cambridge/Improved GFP
    ...the only GFP variant currently listed and characterised in the Registry of Standard Biological Parts is the somewhat outdated GFP mut3b [3] . <br> ...anced versions of GFP – in standard BioBrick format - to the Registry of Standard Biological Parts would thus be expected to have a range of positive effects
    12 KB (1,879 words) - 03:48, 30 October 2008
  • Team:Hawaii/Make Competent E. Coli
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts].
    7 KB (1,158 words) - 23:54, 30 May 2008
  • Team:Hawaii/Protocols/Competent Cells
    ...sse '464 patent]] describes using this buffer for DH5&alpha; cells. The [[Media:pat6709852.pdf | Bloom04]] patent describes the use of essentially the same ...ed by [[User:Tk|Tom Knight]] and the [http://partsregistry.org Registry of Standard Biological Parts].
    5 KB (802 words) - 04:22, 24 June 2008
  • Team:IIT Madras/Project
    ...is an essential part of the synthetic biologist's toolkit. The Registry of Standard Biological Parts contains a growing number of promoters whose expression ca ...ements. We are currently characterizing the library of promoters against a standard control, the unmodified Lutz-Bujard LacO promoter.
    7 KB (1,125 words) - 14:42, 27 October 2008
  • Team:Caltech/Project/Oxidative Burst
    The constructs shown in figure 5 were made using the standard assembly method. They were made to test the various regulatory elements and ... arrow indicates induction with AHL at time 0 hr. Error bars represent one standard deviation(n=3) and are too small to be visible on the negative control.]]
    16 KB (2,564 words) - 20:23, 25 October 2008
  • Team:Heidelberg/Project/Killing II
    ...sually are not secreted, whereas colicins of group A are released into the media.[[Team:Heidelberg/Project/Killing_II#References|[4]]], [[Team:Heidelberg/Pr ...ot elucidated so far. Clear is that most of the colicin is released in the media, but only in a late state of release small amounts of lysis proteins were d
    65 KB (9,654 words) - 17:16, 29 October 2008
  • Team:Purdue/Project
    ...lacZ'' has been used for blue/white screening of ''E. coli'' for decades. Standard ''E. coli'' naturally make the lacZ protein (beta-galactosidase, or beta-ga ...ect one. For this project, we are using the ''recA'' promoter, which is a standard promoter of the SOS pathway and is activated only after extreme DNA damage.
    12 KB (1,859 words) - 23:49, 29 October 2008
  • Team:Harvard/Dailybook/Week1/Chemical and Light
    None of the samples in the LB amp media grew. All the others grew and then were miniprepped and stored in glycerol 6/26: Amp cultures still did not grow in liquid media, neither at 1:1000 or a 1:2000 dilution of amp
    34 KB (5,237 words) - 18:54, 26 October 2008
  • Team:Harvard/Dailybook/Week4/Chemical and Light
    8 100μL PCR reactions set up (standard mix): DNA template is S1 P13, 40 cycles, annealing temp is 58°C, extension 8 100μL PCR reactions set up (standard colony mix) w/ WT Shewanella
    38 KB (5,639 words) - 19:03, 26 October 2008
  • Minnesota/23 September 2008
    ...d fragment was cleaned by ethanol precipitation. The size of the reference standard promoter is 35 bp. (was obtained from source plate 1002, well number 10F). ...late (to preserve the individual clones) and inoculated separately into LB media to do plasmid isolation.
    3 KB (487 words) - 20:11, 29 October 2008
  • Team:Bologna/Biosafety
    ...terns in the building. Work is generally conducted on open benchtops using standard practices. Usually, "contaminated" materials are left in open (but separate # the recommended Standard Microbiological Practices, Special Practices, and Safety Equipment for BSL3
    29 KB (4,412 words) - 02:19, 30 October 2008
  • Materials and Methods
    Yeast were transformed using standard procedures (LiOAc method). ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]).
    3 KB (508 words) - 23:50, 29 October 2008
  • Team:TUDelft/Protocols
    * Grow a 5ml overnight culture of cells in LB media. * In the morning, dilute this culture back into 25-50ml of fresh LB media in a 200ml conical flask. You should aim to dilute the overnight culture by
    19 KB (3,279 words) - 23:04, 29 October 2008
  • Team:Harvard/GenProtocols
    #Remove media and wash with 0.33 volumes (330μL) of 1M sorbitol #Grow 5mL culture of bacteria in LB media overnight, 37° C , shaking
    13 KB (2,102 words) - 05:38, 29 October 2008
  • Team:Heidelberg/Notebook/Killing II/11thweek
    ... -> 200 µl Referencepromotercells + 200 µl Receivercells + 600 µl TB media ... -> 200 µl Referencepromotercells + 40 µl Receivercells + 760 µl TB media
    36 KB (4,963 words) - 21:50, 28 October 2008
  • Team:iHKU/protocol
    ... were collected by centrifugation, <em>ca</em>. 900 μl of supernatant media was discarded, and then the resuspended cells were plated onto LB agar con A 50 mL culture of EColi. was grown in 2% LB media overnight (16 hours) in 37’C shaker. A fresh 50 mL LB culture was inocul
    63 KB (6,466 words) - 19:25, 29 October 2008
  • Team:KULeuven/Data/GFP
    ==== Media ==== The media used were Luria-Bertani (LB) medium or ABT minimal medium. LB medium was us
    11 KB (1,747 words) - 15:41, 19 October 2008
  • Team:University of Washington/Project
    ...ry that provided a PoPS output when these three conditions are met. Using standard BioBrick assembly methods, we placed the gene for LuxR under the control of ...design of projects like ours easier using information from the Registry of Standard Biological Parts (see SeToB tab).
    23 KB (3,437 words) - 04:40, 30 October 2008
  • Team:Bologna/Protocols
    ...istilled water-EOH 95%) into the spot that corresponds to the Biobrick™-standard part that you want. # Put 5 ml of LB media in a falcon tube.
    13 KB (2,058 words) - 13:45, 28 October 2008
  • Team:NTU-Singapore/Wetlab/Materials and Equipment
    ==Bacteria strains and media== ...ht growth of cells were Luria-Bertani broth (LB). Cells were diluted in M9 media to an OD600 of 1 before ready to be read under Fluorescence Microplate Read
    6 KB (869 words) - 05:47, 27 October 2008
  • Team:Michigan/Notebook/TransformationProtocol
    == <font color=royalblue size=5> Transformation Standard Protocol </font> == 7. Add 900 &mu;L pre-warmed LB media & allow to recover on spinning wheel in incubator (37 degrees Celsius) for
    2 KB (239 words) - 03:06, 30 October 2008
  • Team:Cambridge/Signaling
    Bacillus strain 1A1 (derivative of standard strain 168) * deficient in tryptophan, have to add to media
    7 KB (1,055 words) - 14:52, 28 October 2008
  • Team:Cambridge/Signalling
    Bacillus strain 1A1 (derivative of standard strain 168) * deficient in tryptophan, have to add to media
    6 KB (848 words) - 04:26, 30 October 2008
  • Team:ETH Zurich/Project/Background
    ...ticellularity or a nucleus can live a happy life with even less genes. The standard laboratory bacterium Escherichia coli is happy with just 4,467 genes (5, 6) ...s biosynthetic pathways that are not needed for growth in standard glucose media and that therefore only add to the costs of protein synthesis without incre
    9 KB (1,351 words) - 09:58, 30 October 2008
  • Everything you ever wanted to know about AarI
    ...lves the biggest problem faced when trying to do multipart ligations using standard restriction enzymes, the self ligation of a part, blocking it's incorporati ...serts). While parts can be made with any 4 base overhang (end), we chose a standard set, termed A, B, C, and D. This allows parts to be traded between research
    6 KB (1,025 words) - 04:20, 29 October 2008
  • Team:Bologna/Notebook
    ...rk: week by week you can find all the procedures, links to the registry of standard parts and protocols. The chronological structure of this section, organized ... to test and set up the new [http://partsregistry.org/Measurement Biobrick Standard Measurement Protocol]
    15 KB (2,315 words) - 01:13, 30 October 2008
  • Team:Rice University/RESULTS
    ...nd the consumption of ''p''-coumaric acid, which will only be added to the media for the 4CL::STS-integrated yeast; the 4CL::STS+TAL will produce resveratro ...b|700px|'''Figure C1. Example HPLC chromatogram of a ''trans''-resveratrol standard.''']]
    7 KB (962 words) - 05:22, 30 October 2008
  • Team:Johns Hopkins/Notebook/GROUP 3: Short two way stops
    [[Media:10-4-08 digest of 10;11;13.jpg]] [[Media:10-04-08 PCR verification.jpg]]
    7 KB (1,012 words) - 01:06, 29 October 2008
  • Team:Hawaii/Project/Part B MaterialsMethodsResults
    ...pilA'' and ''slr2016'' secretion signal sequences were syntheized with the standard Biobrick sites. Oligonucleotide fragments of each were hybridized with its Following standard PCR protocol, [http://www.partsregistry.org/Part:BBa_E0040 GFP] was convert
    13 KB (1,867 words) - 03:04, 30 October 2008
  • /Project/Vitamins
    ...nating in a double stop (TAATAA) sequence, as regulated by the Registry of Standard Biological Parts. ...e'' given known quantities of folate present in the growth media. Once the standard curve has been established, then experimental growth levels, as quantified
    10 KB (1,460 words) - 17:32, 22 August 2008
  • Team:Bologna/Software
    ...h segmented bacteria the software computes the size in pixel, the mean and standard deviation of 
intensity florescence. The use of the software is easy and ...of pixels) and obtain informations about their area, fluorescence mean and standard deviation. Fluorescence is read from R channel for RFP, G for GFP and B for
    6 KB (826 words) - 11:10, 27 April 2016
  • Team:Heidelberg/Notebook/Killing I/Notebook/week8
    * preparation of in vitro packaging media === Cloning oriT in standard plasmid ===
    27 KB (3,801 words) - 11:47, 29 October 2008
  • Main Page
    ...oree? See the <a class="pink" href="https://2008.igem.org/Jamboree/Media">Media</a> page for pictures and videos. Post your own links to let us know where **[[Jamboree/Media | Media ]]<small>(pictures & fun videos!)</small>
    10 KB (1,324 words) - 20:30, 5 May 2016
  • Team:Illinois/Antibody GPCR Fusion
    ...of the FUS1-Fluo.Prot. fusion into the yeast genome, which seems to be the standard for this type of thing. *Preparation of Yeast Media [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/article/m
    21 KB (3,227 words) - 02:19, 24 October 2008
  • Team:Hawaii/Project/Part B
    ...A'' and ''slr2016'' secretion signal sequences will be syntheized with the standard Biobrick sites. Oligonucleotide fragments of each will be hybridized with i ...efficacy of the signal peptides in transporting GFP into the extracellular media can be measured using a spectrofluorometer.
    11 KB (1,558 words) - 02:25, 29 October 2008
  • Team:University of Chicago/Notebook/Norayucel
    7. Added 250microliters SOC media (prepared at 1:30)<br> ...0microliters for pGreen, 1mL for E0240 (scaled up to accommodate for extra media dilution)
    11 KB (1,571 words) - 16:42, 28 September 2008
  • Team:EPF-Lausanne/Notebook
    ===Media and buffers=== ..., animals or the environment, other than the ones which are encountered in standard laboratory work with ''E.coli''. Therefore, we estimate the risk to researc
    16 KB (2,322 words) - 23:27, 29 October 2008
  • Team:Heidelberg/Human Practice/Open Day
    ...translated the text in english, the original german version can be found [[Media:IGEM-Veranstaltung_Heidelberg.pdf|'''here''']]). ...en_Day_english.ppt.html here] (rapdishare download file) and as pdf-file [[Media:Open_Day_english.pdf| here]]. <br/>
    60 KB (9,576 words) - 15:58, 29 October 2008
  • Team:UCSF/Materials and Methods
    ...gal2::NatR, allowing graded activation of galactose inducible promoters ([[Media:Hawkins and Smolke.pdf]]). ...ere grown to early log phase (OD600=0.1-0.3) in complete synthetic dropout media and then treated with 1 μM α-factor (Zymo Research) to activate the pathw
    3 KB (499 words) - 03:27, 30 October 2008
  • Team:Alberta NINT/Project
    [[Media:NINT_PlasmidDesignAndConstruction.pdf|Plasmid Design and Construction]] ...ion in the absence of any input. The y-axis is shown in Miller units (the standard for such assays).
    21 KB (3,119 words) - 16:44, 30 March 2009
  • Team:Hawaii/Member Training
    ## Read [[Image:Idempotent Vector Design for Standard Assembly of Biobricks.pdf|Biobrick Technical Manual]], don't worry about un ## [http://igem.blip.tv/file/254379 Drew Endy: What is a Standard Biological Part] 2 minute 31 seconds
    2 KB (309 words) - 12:16, 31 May 2008
  • Team:UC Berkeley/Protocols
    # Plate using the sterilized glass-bead method or with standard spreaders. # Plate using the sterilized glass-bead method or with standard spreaders.
    17 KB (2,857 words) - 22:03, 27 October 2008

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