Team:Bay Area RSI/Project

From 2008.igem.org

(Difference between revisions)
(Targeting Infarcted Cardiac Tissue)
(Cardiomyocyte Differentiation Circuit)
Line 99: Line 99:
-
== '''''Cardiomyocyte Differentiation Circuit'''''==
+
== '''''Use As a Novel Therapy For MI Patients '''''==
 +
Current therapies and problems
 +
Diff types of MI and need for better solution
Therpeautic approach with combined circuitry
Therpeautic approach with combined circuitry
use in tandem, synergistic application
use in tandem, synergistic application
== Results ==
== Results ==

Revision as of 19:33, 27 October 2008


This is a template page. READ THESE INSTRUCTIONS.
You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples HERE.
You MUST have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace.



You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
Example logo.png

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Your team picture
Team Example 2


Home The Team The Project Parts Submitted to the Registry Modeling Notebook

(Or you can choose different headings. But you must have a team page, a project page, and a notebook page.)


Contents

Project Overview

Every year over 1.2 million people suffer myocardial infarction. The resulting heart damage requires new approaches for effective repair. Stem cell therapies provide hope. However none of the stem cell therapies currently in clinical trials addresses the need for efficient stem cell targeting to cardiac tissue or the need to replace efficiently dead tissue with new cardiomyocytes. To address these problems, we have built several genetic circuits that work sequentially to repair the heart. First, we have built an inducible differentiation circuit that closely resembles the endogenous differentiation pathway, to program cells to become cardiomyocytes. Second, we have built circuits that use the extracellular domains of chimeric proteins to target cells to damaged cardiac tissue. Upon binding, novel receptor-coupled intein-mediated signaling domains activate effector genes that then aid in integration, inhibition of cell death, and the alteration of the tissue microenvironment.

Targeting Infarcted Cardiac Tissue

C-Reactive Protein Receptor Intein Mediated Signaling Circuit

CRP Signaling System diagram.jpg Continuation from last year Circuit overview construct image

FCGamma Receptor Binds Immobilised CRP on Damaged Cardiomyocytes In Vitro

description picture picture exp

CRP activates GFP signaling upon FCGamma Binding

picture picture exp

Generation of Clonal lines of FCGamma+ rat H9C2 Cardiomyocytes

pic + explanation

Effectors To Aid in integration, anti-apoptosis, and the alteration of the tissue microenvironment

Overview Construct image pics from slides

Differentiation Circuit Concerns

Future Directions

Sensor sensitivity-> , avidity, affinity More universal receptor --> 2nd gen SCFV to target other tissue

Cardiomyocyte Differentiation Circuit

endogenous pathways pathway overview construct image + explanation data


Differentiation Circuit Concerns

CA, efficiency, transdiff,

Future Directions

inducible, non-SC progenitor, native cardiofiobroblast


Use As a Novel Therapy For MI Patients

Current therapies and problems Diff types of MI and need for better solution Therpeautic approach with combined circuitry use in tandem, synergistic application

Results