Team:Bologna/Notebook
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HOME | PROJECT | TEAM | SUBMITTED PARTS | MODELING | WETLAB | LAB-BOOK | BIOSAFETY AND PROTOCOLS |
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Under notebook are all relevant information regarding the work done every week in the laboratory and protocols followed.
Protocols
Protocol | Biosafety level |
Plates preparation | |
Amplification | |
Transformation | |
Inoculation | |
Miniprep | |
Digestion reaction | |
Gel preparation | |
Electrophoretic run | |
Gel extraction | |
Ligation reaction | |
Chemiocompetent cells | |
Mediums and buffers | |
Antibiotics stocks preparation | |
IPTG stocks preparation | |
Fluorescence test | |
M9 supplemented media | |
Week 1: from 07/21/08 to 07/27/08
General Preparations
- Preparation of chemiocompetent cells from E. Coli DH5α, Top10 and DB 3.1
- Preparation of antibiotic stocks for Ampicillin and Kanamicin
- Preparation of LB medium and LB plates for cloning.
Week 2: from 07/28/08 to 08/03/08
1. Eluition and Amplification from 2008 Registry Collection: R0082, R0083, M30109 in TOP10 strain to build and characterize the Light response system to be our spatial selective trigger.
2. Eluition and Amplification from 2008 Registry Collection: E0240, pSB3K3_P1010in DB3.1 and the Practice Promoter Set (J23150, J23151, J23102) to test and set up the new Biobrick Standard Measurement Protocol
Week 3: from 08/04/08 to 08/10/08
Week 4: from 08/11/08 to 08/17/08
Week 5: from 08/18/08 to 08/24/08
Week 6: from 08/25/08 to 08/31/08
Week 7: from 09/01/08 to 09/07/08
Week 8: from 09/08/08 to 09/14/08
Week 9: from 09/15/08 to 09/21/08
Week 10: from 09/22/08 to 09/28/08
Week 11: from 09/29/08 to 10/05/08
Week 12: from 10/06/08 to 10/12/08
Week 13: from 10/13/08 to 10/19/08
Week 14: from 10/20/08 to 10/26/08
Week 15: from 10/27/08 to 10/29/08