"
Team:Caltech/Protocols/FimE
From 2008.igem.org
(Difference between revisions)
| Line 8: | Line 8: | ||
# Grow 5mL LB cultures with appropriate antibiotics overnight. | # Grow 5mL LB cultures with appropriate antibiotics overnight. | ||
# Backdilute culture 1:100 into fresh 5mL LB with appropriate antibiotics. | # Backdilute culture 1:100 into fresh 5mL LB with appropriate antibiotics. | ||
| - | # When the cultures reach 0.4 OD at 600nm, add arabinose and grow at 37C for 1 hour. | + | # When the cultures reach 0.4 OD at 600nm, add 0.1% arabinose and grow at 37C for 1 hour. |
| - | # Wash and resuspend cells in phosphate buffer solution. | + | # Wash and resuspend cells in phosphate buffer solution (100mM, 7.0pH). |
| - | # Measure GFP fluorescence on plate reader. | + | # Measure GFP fluorescence of 200 uL of culture at 498nm on plate reader. |
}} | }} | ||
Latest revision as of 05:25, 24 October 2008
|
People
|
FimE Inversion Assay
  |
