Team:Caltech/Protocols/MIC Assay
From 2008.igem.org
(Difference between revisions)
(2 intermediate revisions not shown) | |||
Line 7: | Line 7: | ||
<br> | <br> | ||
- | + | # Grow one 5 ml culture to saturation at 37C with shaking in the indicated media and antibiotics as necessary. | |
+ | # Back dilute parent culture 1:50 into 5 ml of the same media and antibiotics plus differing amounts of hydrogen peroxide and let shake at 37C overnight. | ||
+ | # Record MIC as the concentration of hydrogen peroxide necessary to prevent overnight turbid growth. | ||
}} | }} |
Latest revision as of 00:14, 30 October 2008
People
|
Measuring Minimum Inhibitory Concentration
|