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Team:Chiba/AHL Receiver Phase
From 2008.igem.org
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===Experiment=== | ===Experiment=== | ||
[[Image:R-family-crosstalk-test Chiba.gif|frame|right|Fig.R family crosstalk test]] | [[Image:R-family-crosstalk-test Chiba.gif|frame|right|Fig.R family crosstalk test]] | ||
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| + | *'''Sender''' | ||
| + | **[http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL autoinucer)] | ||
| + | [[Image:LuxI-sender Chiba.gif|left|]] | ||
===Result=== | ===Result=== | ||
Revision as of 11:09, 28 October 2008
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Signal Molecule Receiver Phase
Quorum_Sensing_Crosstalk
Design
Table.1 LuxR family gene
センダーとレシーバーを元来とは異なる組み合わせにすることによって、thresholdに達する時間を遅くする。（杉山）
Experiment
Fig.R family crosstalk test
- Sender
Result
Plasmid_Copy_number
Design
Experiment
Result
LuxR_mutant
Design
Fig.6 Location of the LuxR mutant in LuxR
Table.1 Seisitivity of LuxR mutants. Data modified from Collins et.al. Mol. Microbiol. 55, 712–723 (2005)
Collins et.al. described the hyper-sensitive variants of luxR to AHL.(Collins, C. H., Arnold, F. H. & Leadbetter, J. R. Directed evolution of Vibrio fischeri LuxR for increased sensitivity to a broad spectrum of acyl-homoserine lactones. Mol. Microbiol. 55, 712–723 (2005))
私たちはmutated Receiverを用いることで、AHL感受性の違う２種類（WTと変異体）のレシーバーを用意し、delay-timeのバリエーションを増やした。 (小林)
Experiment
Result
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