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Team:Chiba/AiiA Receiver Phase
From 2008.igem.org
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| - | [[Image:AiiA-Receiver-result-detail Chiba.gif|frame|left|Fig.Time Delay Test]]<br clear=all> | + | [[Image:AiiA-Receiver-result-detail Chiba.gif|frame|left|Fig.Time Delay Test]] |
| + | AiiAを発現させると、GFPの発現自体が極端に減ってしまったので、AHL自体を減らしてしまうと発現量の最大値が小さくなってしまう。 | ||
| + | <br clear=all> | ||
===conclusion=== | ===conclusion=== | ||
Revision as of 10:41, 29 October 2008
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AiiA Receiver
Fig.6 AiiA Receiver
Design
オートインデューサー不活性化酵素であるAiiAをレシーバー菌で発現させることにより、レシーバー菌内でのAHL濃度の増加が遅くなるため、AiiAを発現しないレシーバー菌に比べて、GFP発現が遅くなる。（小林）
Experiment
- Sender
- [http://partsregistry.org/Part:BBa_S03623 BBa_S03623 (AHL autoinucer)]
- Receivers
- AiiA Receiver
- non-AiiA Receiver
result
Fig.Time Delay Test
AiiAを発現させると、GFPの発現自体が極端に減ってしまったので、AHL自体を減らしてしまうと発現量の最大値が小さくなってしまう。
conclusion
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