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Team:Chiba/Calendar-Home/14 September 2008
From 2008.igem.org
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===Team:Communication=== | ===Team:Communication=== | ||
:(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' | :(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' | ||
| + | ::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106] | ||
| + | ::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019] | ||
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:--->(15/9) | :--->(15/9) | ||
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===Team:Output=== | ===Team:Output=== | ||
Revision as of 23:49, 29 October 2008
13 September 2008 <|> 15 September 2008
Laboratory work
Team:Communication
- (13/9)--->Mini prep
- Transformation
- Competent cells : XL10Gold
- BBa_T9002(2007)
- BBa_K084009(Plac+RhlI+LVA)
- BBa_K084010(Plac+CinI+LVA)
- --->(15/9)
Team:Output
- BBa_Q04510
- BBa_I716463
- BBa_C0051
- BBa_C0070
- BBa_J06620
- BBa_J22141
- BBa_C0076
- BBa_I716452
- BBa_I716462
- BBa_C0078
- BBa_J01101
- BBa_C0071
- BBa_I712019
- BBa_S01416
- BBa_C0079
- BBa_C0077
- BBa_J23007
- BBa_I7100(×2)
- BBa_S01416
| Sample No. | each |
| DNA(digested sample)(μL) | 3 |
| Buffer2(x10)(μL) | 1 |
| dH2O(μL) | 6 |
| EcoRI(μL) | 0.075 |
| TOTAL(μL) | 10 |
--> left at 37°C for 1 hour
| Sample No. | each |
| DNA(digestion sample)(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
