"
Team:Chiba/Calendar-Home/14 September 2008
From 2008.igem.org
(Difference between revisions)
(→Team:Communication) |
(→Team:Communication) |
||
| Line 5: | Line 5: | ||
==Laboratory work== | ==Laboratory work== | ||
===Team:Communication=== | ===Team:Communication=== | ||
| - | + | ||
| + | (13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' | ||
::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106] | ::*[http://partsregistry.org/Part:BBa_I7106 BBa_I7106] | ||
::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019] | ::*[http://partsregistry.org/Part:BBa_I23019 BBa_I23019] | ||
| + | (13/9)--->[[https://2008.igem.org/Team:Chiba/protocol/ligation/dephosphorylation|SAP]] | ||
| + | ::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500] | ||
| + | ::*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] | ||
| + | :--->(19/9) | ||
| - | + | '''[[Team:Chiba/protocol/transformation|Transformation]]''' | |
:Competent cells : XL10Gold | :Competent cells : XL10Gold | ||
::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007) | ::*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002](2007) | ||
| Line 17: | Line 22: | ||
::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA) | ::*[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084010 BBa_K084010](Plac+CinI+LVA) | ||
| - | :--->(15/9) | + | :--->(15/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]''' |
===Team:Output=== | ===Team:Output=== | ||
Revision as of 23:55, 29 October 2008
13 September 2008 <|> 15 September 2008
Laboratory work
Team:Communication
(13/9)--->Mini prep
(13/9)--->[[1]]
- --->(19/9)
- Competent cells : XL10Gold
- BBa_T9002(2007)
- BBa_K084009(Plac+RhlI+LVA)
- BBa_K084010(Plac+CinI+LVA)
- --->(15/9)Gel Check
Team:Output
- BBa_Q04510
- BBa_I716463
- BBa_C0051
- BBa_C0070
- BBa_J06620
- BBa_J22141
- BBa_C0076
- BBa_I716452
- BBa_I716462
- BBa_C0078
- BBa_J01101
- BBa_C0071
- BBa_I712019
- BBa_S01416
- BBa_C0079
- BBa_C0077
- BBa_J23007
- BBa_I7100(×2)
- BBa_S01416
| Sample No. | each |
| DNA(digested sample)(μL) | 3 |
| Buffer2(x10)(μL) | 1 |
| dH2O(μL) | 6 |
| EcoRI(μL) | 0.075 |
| TOTAL(μL) | 10 |
--> left at 37°C for 1 hour
| Sample No. | each |
| DNA(digestion sample)(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
