Team:Chiba/Calendar-Home/14 September 2008

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(Difference between revisions)
(Team:Communication)
(Laboratory work)
 
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:--->(19/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
:--->(19/9)'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
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'''[[Team:Chiba/protocol/Time Delay Test|Time Delay Test]]'''
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===Team:Output===
===Team:Output===

Latest revision as of 23:59, 29 October 2008

>Home | Notebook

13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep

(13/9)--->[[1]]

--->(19/9)

Transformation

Competent cells : XL10Gold
--->(19/9)Gel Check


Time Delay Test



Team:Output

Time Responce Liquid

Digestion test

Sample No. each
DNA(digested sample)(μL) 3
Buffer2(x10)(μL) 1
dH2O(μL) 6
EcoRI(μL) 0.075
TOTAL(μL) 10

--> left at 37°C for 1 hour

Gel check

Sample No. each
DNA(digestion sample)(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12