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Team:Chiba/Calendar-Home/16 September 2008
From 2008.igem.org
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[[Team:Chiba/protocol/PCR|PCR]] | [[Team:Chiba/protocol/PCR|PCR]] | ||
| - | * | + | *[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003] |
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<tr> | <tr> | ||
<td width="257">Sample No.</td> | <td width="257">Sample No.</td> | ||
| - | <td> | + | <td>[http://partsregistry.org/wiki/index.php?title=Part:BBa_K084003 BBa_K084003]</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
Revision as of 22:29, 29 October 2008
15 September 2008 <|> 17 September 2008
Laboratory work
Team:Communication
time-delay test with (15/9) cultures.
- reaction temparature was 37°C and 30°C.
- result
- Created Glycerol Stocks from (15/9) cultures.
Team:Output
Digestion test
- BBa_R0040(ptet)+BBa_S03158(RBS+RhlR)(×2)
- BBa_R0040(ptet)+BBa_S03156(RBS+lasR)(×5)
- BBa_R0040(ptet)+Ba_S03160(RBS+cinR)(×2)
- BBa_S03158(RBS+RhlR)
- BBa_S03156(RBS+lasR)
- BBa_S03160(RBS+cinR)
| Sample No. | each |
| DNA(μL) | 3 |
| EcoRI(μL) | 0.083 |
| PstI(μL) | 0.083 |
| BSA(×10)(μL) | 1 |
| Buffer3(×10)(μL) | 1 |
| dH2O(μL) | 5 |
| TOTAL(μL) | 10 |
-->37°C 1 hour
| Sample No. | each |
| DNA(μL) | 10 |
| Loading Dye(μL) | 2 |
| TOTAL(μL) | 12 |
- BBa_J37019(LuxI+RBS+LuxR)->no colony
- BBa_J37033(RBS+LuxR)
- BBa_J37020(LuxI+LuxR+pLux+GFP)
- BBa_J37032(pLux+GFP)
| Sample No. | BBa_K084003 |
| DNA(μL) | 1 |
| Venus Fwd(μL) | 5 |
| Rev(VR)(μL) | 5 |
| Thermo pol Buffer(μL) | 10 |
| dNTP mix(μL) | 10 |
| Vent(μL) | 1 |
| dH2O(μL) | 68 |
| TOTAL(μL) | 100 |
