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Team:Chiba/Calendar-Home/17 August 2008
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===Output team=== | ===Output team=== | ||
バイオブリックパーツおこし | バイオブリックパーツおこし | ||
| - | BBa_I73002 | + | <br>BBa_I73002 |
| - | BBa_I73004 | + | <br>BBa_I73004 |
| - | BBa_I73003 | + | <br>BBa_I73003 |
| - | BBa_I763026 | + | <br>BBa_I763026 |
| - | + | <br>BBa_E0430 | |
| - | Elution :EB buffer 5μL -> Spin(15000rpm,3Min.) | + | <br>BBa_E0420 |
| + | <br>BBa_J60720 | ||
| + | <br>BBa_S01416 | ||
| + | <br>Elution :EB buffer 5μL -> Spin(15000rpm,3Min.) | ||
Insert PCR | Insert PCR | ||
Revision as of 15:35, 18 August 2008
Output team
バイオブリックパーツおこし
BBa_I73002
BBa_I73004
BBa_I73003
BBa_I763026
BBa_E0430
BBa_E0420
BBa_J60720
BBa_S01416
Elution :EB buffer 5μL -> Spin(15000rpm,3Min.)
Insert PCR
DNA template 1μL
Fwd primer(VF II) 5μL
Rev primer(VR) 5μL
Termo pol buffer 10μL
Vent pol 1μL
dH2O 68μL
==========================
Total:100μL
95℃(5min.) -> [95℃(30sec.) -> 53℃(1min.) -> 72℃(2min.)]x25cycle -> 72℃(10min.) -> store
Gel check
