Team:Chiba/Calendar-Home/18 August 2008

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(Difference between revisions)
(→Team:communication)
(→Team:communication)
 
Line 23: Line 23:
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">DNA Template</td>
+
<td width="257">DNA Template(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dNTP mix</td>
+
<td>dNTP mix(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Foward Primer</td>
+
<td>Foward Primer(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Reverse Primer</td>
+
<td>Reverse Primer(&mu;L)</td>
<td>5</td>
<td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA polymerase</td>
+
<td>DNA polymerase(&mu;L)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Thermopol Buffer</td>
+
<td>Thermopol Buffer(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>68</td>
<td>68</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>100μL</td>
+
<td>100</td>
</tr>
</tr>
</table>
</table>
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</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>7</td>
<td>7</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
-
<td>12μL</td>
+
<td>12</td>
</tr>
</tr>
</table>
</table>

Latest revision as of 05:13, 30 October 2008

>Home | Notebook

17 August 2008 <|> 19 August 2008

Contents

Laboratory work

Team:Input

Transformation

competent cells : XL10Gold(Cm),LB-agar(KanR)

Team:communication

PCR


DNA Template(μL) 1
dNTP mix(μL) 10
Foward Primer(μL) 5
Reverse Primer(μL) 5
DNA polymerase(μL) 1
Thermopol Buffer(μL) 10
dH2O(μL) 68
TOTAL(μL) 100


95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C


--->Gel Check


Chiba-0818.JPG
Sample DNA 3
Loading Dye(μL) 2
dH2O(μL) 7
TOTAL(μL) 12
From left;



Transformation

competent cells : XL10G


-->(20/8)Mini prep

Team:Output

no work

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