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Team:Chiba/Calendar-Home/18 September 2008
From 2008.igem.org
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[[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] | [[Team:Chiba/protocol/phenotype/timedelay/j|time-delay test]] | ||
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*conditions | *conditions | ||
#strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br> | #strain<br>Senders:XL10GOLD<br>Receiver:JW1908<br> | ||
Revision as of 12:37, 26 October 2008
17 September 2008 <|> 19 September 2008
Laboratory work
Team:Communication
- conditions
- strain
Senders:XL10GOLD
Receiver:JW1908
- temparature
37℃ and 30℃
Team:Output
SAP 1μL
->left for:
- 1 hour at 37 degrees
- 30 minutes at 65 degrees.
->eluted with 25μL of NFW.
- vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
- insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
- Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
| Sample No. | Vector&insert | Vector |
| DNA(vector)(μL) | 3.5 | 3.5 |
| DNA(insert)(μL) | 7.5 | - |
| Ligase(μL) | 1 | 1 |
| Ligase Buffer(μL) | 4 | 4 |
| dH2O(μL) | 4 | 4 |
| TOTAL(μL) | 20 | 20 |
- [http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
- [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]
- Ligation product(Venus YFP)
- [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
- [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
- [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
| Sample No. | each |
| DNA(μL) | each |
| Loading Dye(μL) | 1 |
| dH2O(μL) | 4 |
| TOTAL(μL) | 6 |
