Team:Chiba/Calendar-Home/19 August 2008

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18 August 2008 <|> 20 August 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

PCR

BBa_I9026(2007)
BBa_I9026(2006)
BBa_I9030(2007)
BBa_I9030(2006)
BBa_S3154(2007)
BBa_S3154(2006)
BBa_F2620(2007)･･･control

DNA Template	1
dNTP mix(μL)	10
Foward Primer(μL)	5
Reverse Primer(μL)	5
DNA polymerase VENT(μL)	1
Thermopol Buffer(μL)	5
dH₂O(μL)	28
TOTAL(μL)	50

95°C,5min -> ( 95°C,1min -> 52°C,1min -> 72°C,1min )･･･25cycles -> 72°C,10min -> 6°C

--->Gel Check

Sample DNA	3
Loading Dye(μL)	2
dH₂O(μL)	7
TOTAL(μL)	12

From left;

BBa_I9026(2007) ->None
BBa_I9026(2006) ->None
BBa_I9030(2007) ->None
BBa_I9030(2006) ->None
BBa_S3154(2007) ->None
BBa_S3154(2006) ->None
BBa_F2620(2007)･･･control ->OK

Team:Output

no work

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