Team:Chiba/Calendar-Home/23 August 2008

From 2008.igem.org

(Difference between revisions)
(Team:Communication)
(Team:Communication)
 
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[[Team:Chiba/Calendar-Home/22 August 2008|22 August 2008 <]]|[[Team:Chiba/Calendar-Home/24 August 2008|> 24 August 2008]]
[[Team:Chiba/Calendar-Home/22 August 2008|22 August 2008 <]]|[[Team:Chiba/Calendar-Home/24 August 2008|> 24 August 2008]]
 +
 +
== Meeting with UmeG ==
 +
'''the ppt for our weekly meeting(japanese)'''
 +
#Team:Input
 +
#[[media:0823-soujushin-1.ppt|Team:Communication]]
 +
#[[media:Output_chiba_080823.ppt|team:Output]]
 +
==Laboratory work==
==Laboratory work==
Line 33: Line 40:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(&mu;L)</td>
<td>3</td><td>1</td><td>3</td><td>3</td>
<td>3</td><td>1</td><td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI(&mu;L)</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstI(&mu;L)</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer EcoRⅠ</td>
+
<td>Buffer EcoRI(&mu;L)</td>
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
<td>-</td><td>-</td><td>1</td><td>1</td>
<td>-</td><td>-</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>-</td><td>-</td><td>5</td><td>5</td>
<td>-</td><td>-</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>3</td><td>1</td><td>10</td><td>10</td>
<td>3</td><td>1</td><td>10</td><td>10</td>
</tr>
</tr>
Line 70: Line 77:
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>1~6</td><td>7</td>
<td>1~6</td><td>7</td>
</tr>
</tr>
Line 78: Line 85:
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td><td>1</td>
<td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>2</td><td>4</td>
<td>2</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td><td>6</td>
<td>6</td><td>6</td>
</tr>
</tr>
</table>
</table>
:From left; Sanple No. 1~7
:From left; Sanple No. 1~7
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+
::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+
::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+
::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+
::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+
::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+
::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
|}
|}
Line 109: Line 116:
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>8~21</td>
<td>8~21</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>10</td>
<td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>2</td>
<td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>12</td>
<td>12</td>
</tr>
</tr>
Line 128: Line 135:
:From left;
:From left;
:(Single Digestion) Sanple No. 8~14
:(Single Digestion) Sanple No. 8~14
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
+
::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
+
::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
+
::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
:(Double Digestion) Sample No. 15~21
:(Double Digestion) Sample No. 15~21
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
+
::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
+
::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
+
::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?
+
::*[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?
|}
|}
Line 150: Line 157:
:'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
:'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
-
::BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]
+
::[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>1</td><td>2</td><td>3</td>
<td>1</td><td>2</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Sample DNA</td>
+
<td>Sample DNA(&mu;L)</td>
<td>3</td><td>3</td><td>3</td>
<td>3</td><td>3</td><td>3</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI(&mu;L)</td>
<td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI(&mu;L)</td>
<td>-</td><td>-</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ Buffer</td>
+
<td>EcoRI Buffer(&mu;L)</td>
<td>-</td><td>0.9</td><td>0.8</td>
<td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BSA</td>
+
<td>BSA(&mu;L)</td>
<td>-</td><td>1</td><td>1</td>
<td>-</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>-</td><td>5</td><td>5</td>
<td>-</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>3</td><td>10</td><td>10</td>
<td>3</td><td>10</td><td>10</td>
</tr>
</tr>
Line 194: Line 201:
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>1</td><td>2</td><td>3</td>
<td>1</td><td>2</td><td>3</td>
</tr>
</tr>
Line 202: Line 209:
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(&mu;L)</td>
<td>1</td><td>2</td><td>2</td>
<td>1</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(&mu;L)</td>
<td>2</td><td>-</td><td>-</td>
<td>2</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(&mu;L)</td>
<td>6</td><td>12</td><td>12</td>
<td>6</td><td>12</td><td>12</td>
</tr>
</tr>
</table>
</table>
:From left;
:From left;
-
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
-
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK  
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK  
-
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad
|}
|}
===Team:Output===
===Team:Output===
no work
no work

Latest revision as of 06:02, 30 October 2008

>Home | Notebook

22 August 2008 <|> 24 August 2008

Contents

Meeting with UmeG

the ppt for our weekly meeting(japanese)

  1. Team:Input
  2. Team:Communication
  3. team:Output


Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)


Sample No. 1- 671 - 71 - 7
DNA(μL) 3133
EcoRI(μL) --0.10.1
PstI(μL) --0.10.1
Buffer EcoRI(μL) --0.90.8
BSA(μL) --11
dH2O(μL) --55
TOTAL(μL) 311010


--->Gel Check
Chiba-0823-1.JPG
Sample No. 1~67
Sample DNA 31
Loading Dye(μL) 11
dH2O(μL) 24
TOTAL(μL) 66
From left; Sanple No. 1~7
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)



Chiba-0823-2.JPG
Sample No. 8~21
Sample DNA(μL) 10
Loading Dye(μL) 2
TOTAL(μL) 12
From left;
(Single Digestion) Sanple No. 8~14
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
(Double Digestion) Sample No. 15~21
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?



Digestion test
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
Sample No. 123
Sample DNA(μL) 333
EcoRI(μL) -0.10.1
SpeI(μL) --0.1
EcoRI Buffer(μL) -0.90.8
BSA(μL) -11
dH2O(μL) -55
TOTAL(μL) 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No. 123
Sample DNA 31010
Loading Dye(μL) 122
dH2O(μL) 2--
TOTAL(μL) 61212
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad

Team:Output

no work