Team:Chiba/Calendar-Home/23 August 2008

From 2008.igem.org

(Difference between revisions)
(New page: >Home | Notebook 22 August 2008 <|> 24 August 2008 ==Labora...)
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===Team:Communication===
===Team:Communication===
:(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
:(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+
::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+
::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+
::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+
::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+
::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+
::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
+
::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
+
::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+
::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+
::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+
::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+
::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
+
::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
:<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
:<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="270">Sample No</td>
+
<td width="270">Sample No.</td>
-
<td> 1</td><td> 2</td><td> 3</td><td> 4</td><td> 5</td><td> 6</td><td> 7</td><td> 8</td><td> 9</td><td>10</td><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td><td>21</td>
+
<td>1- 6</td><td>7</td><td>1 - 7</td><td>1 - 7</td>
-
</tr><tr>
+
-
<td>BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]</td>
+
-
<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]</td>
+
<td>DNA</td>
-
<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
+
<td>3</td><td>1</td><td>3</td><td>3</td>
</tr>
</tr>
-
<tr>
 
-
<td>BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]</td>
 
-
<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<tr>
 
-
<td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td></tr>
 
-
<tr>
 
-
<td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td></tr>
 
-
<tr>
 
-
<td>BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td></tr>
 
<tr>
<tr>
<td>EcoRⅠ</td>
<td>EcoRⅠ</td>
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
<td>PstⅠ</td>
<td>PstⅠ</td>
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
<td>Buffer EcoRⅠ</td>
<td>Buffer EcoRⅠ</td>
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td>
+
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
<tr>
<tr>
<td>BSA</td>
<td>BSA</td>
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
<td>-</td><td>-</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
<td>dH2O</td>
<td>dH2O</td>
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
+
<td>-</td><td>-</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>1</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
<td>3</td><td>1</td><td>10</td><td>10</td>
</tr>
</tr>
</table>
</table>
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::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
|}
|}
-
 
-
 
===Team:Output===
===Team:Output===
no work
no work

Revision as of 09:49, 24 October 2008

>Home | Notebook

22 August 2008 <|> 24 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep


--->Digestion test
  1. BBa_S03154(2007)
  2. BBa_S03154(2006)
  3. BBa_I9026(2007)
  4. BBa_I9026(2006)
  5. BBa_I9030(2007)
  6. BBa_I9030(2006)
  7. BBa_J04500(2007)


Sample No. 1- 671 - 71 - 7
DNA 3133
EcoRⅠ --0.10.1
PstⅠ --0.10.1
Buffer EcoRⅠ --0.90.8
BSA --11
dH2O --55
TOTAL 311010


--->Gel Check
Chiba-0823-1.JPG
Sample No 1~67
Sample DNA 31
Loading Dye 11
dH2O 24
TOTAL 66
From left; Sanple No. 1~7
  • BBa_S03154(2007) [1]
  • BBa_S03154(2006) [2]
  • BBa_I9026(2007) [3]
  • BBa_I9026(2006) [4]
  • BBa_I9030(2007) [5]
  • BBa_I9030(2006) [6]
  • BBa_J04500(2007) [7]



Chiba-0823-2.JPG
Sample No 8~21
Sample DNA 10
Loading Dye 2
TOTAL 12
From left;
(Single Digestion) Sanple No. 8~14
  • BBa_S03154(2007) [8] -> OK
  • BBa_S03154(2006) [9] -> OK
  • BBa_I9026(2007) [10] -> OK
  • BBa_I9026(2006) [11] -> OK
  • BBa_I9030(2007) [12] -> OK
  • BBa_I9030(2006) [13] -> OK
  • BBa_J04500(2007) [14] -> OK
(Double Digestion) Sample No. 15~21
  • BBa_S03154(2007) [15] -> OK
  • BBa_S03154(2006) [16] -> OK
  • BBa_I9026(2007) [17] -> OK
  • BBa_I9026(2006) [18] -> OK
  • BBa_I9030(2007) [19] -> OK
  • BBa_I9030(2006) [20] -> OK
  • BBa_J04500(2007) [21] -> OK?



Digestion test
BBa_J04500(2007:old-sample)[22]
Sample No 123
Sample DNA 333
EcoRⅠ -0.10.1
SpeⅠ --0.1
EcoRⅠ Buffer -0.90.8
BSA -11
dH2O -55
TOTAL 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No 123
Sample DNA 31010
Loading Dye 122
dH2O 2--
TOTAL 61212
From left;
  1. BBa_J04500(2007) [23] -> OK
  2. BBa_J04500(2007) [24]Single Digestion -> OK
  3. BBa_J04500(2007) [25]Double Digestion -> Bad

Team:Output

no work

Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/23_August_2008"