Team:Chiba/Calendar-Home/23 August 2008

From 2008.igem.org

(Difference between revisions)
(Team:Communication)
(Laboratory work)
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[[Team:Chiba/Calendar-Home/22 August 2008|22 August 2008 <]]|[[Team:Chiba/Calendar-Home/24 August 2008|> 24 August 2008]]
[[Team:Chiba/Calendar-Home/22 August 2008|22 August 2008 <]]|[[Team:Chiba/Calendar-Home/24 August 2008|> 24 August 2008]]
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== Meeting with UmeG ==
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'''the ppt for our weekly meeting(japanese)'''
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#Team:Input
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#[[media:0823-soujushin-1.ppt|Team:Communication]]
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#[[media:Output_chiba_080823.ppt|team:Output]]
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==Laboratory work==
==Laboratory work==

Revision as of 03:15, 25 October 2008

>Home | Notebook

22 August 2008 <|> 24 August 2008

Contents

Meeting with UmeG

the ppt for our weekly meeting(japanese)

  1. Team:Input
  2. Team:Communication
  3. team:Output


Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)


--->Digestion test
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)


Sample No. 1- 671 - 71 - 7
DNA 3133
EcoRⅠ --0.10.1
PstⅠ --0.10.1
Buffer EcoRⅠ --0.90.8
BSA --11
dH2O --55
TOTAL 311010


--->Gel Check
Chiba-0823-1.JPG
Sample No. 1~67
Sample DNA 31
Loading Dye 11
dH2O 24
TOTAL 66
From left; Sanple No. 1~7
  1. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
  2. [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
  3. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
  4. [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
  5. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
  6. [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
  7. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)



Chiba-0823-2.JPG
Sample No. 8~21
Sample DNA 10
Loading Dye 2
TOTAL 12
From left;
(Single Digestion) Sanple No. 8~14
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
(Double Digestion) Sample No. 15~21
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) -> OK
  • [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) -> OK
  • [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) -> OK
  • [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK?



Digestion test
[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007:old-sample)
Sample No. 123
Sample DNA 333
EcoRⅠ -0.10.1
SpeⅠ --0.1
EcoRⅠ Buffer -0.90.8
BSA -11
dH2O -55
TOTAL 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No. 123
Sample DNA 31010
Loading Dye 122
dH2O 2--
TOTAL 61212
From left;
  1. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) -> OK
  2. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Single Digestion -> OK
  3. [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)Double Digestion -> Bad

Team:Output

no work