Team:Chiba/Calendar-Home/23 August 2008

From 2008.igem.org

(Difference between revisions)
(→Laboratory work)
(→Team:Communication)
Line 44: Line 44:
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>PstI</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Buffer EcoRⅠ</td>
+
<td>Buffer EcoRI</td>
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
<td>-</td><td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
Line 65: Line 65:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>3</td><td>1</td><td>10</td><td>10</td>
+
<td>3μl</td><td>1μl</td><td>10μl</td><td>10μl</td>
</tr>
</tr>
</table>
</table>
Line 94: Line 94:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td><td>6</td>
+
<td>6μl</td><td>6μl</td>
</tr>
</tr>
</table>
</table>
Line 129: Line 129:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>12</td>
+
<td>12μl</td>
</tr>
</tr>
</table>
</table>
Line 169: Line 169:
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>EcoRI</td>
<td>-</td><td>0.1</td><td>0.1</td>
<td>-</td><td>0.1</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SpeⅠ</td>
+
<td>SpeI</td>
<td>-</td><td>-</td><td>0.1</td>
<td>-</td><td>-</td><td>0.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ Buffer</td>
+
<td>EcoRI Buffer</td>
<td>-</td><td>0.9</td><td>0.8</td>
<td>-</td><td>0.9</td><td>0.8</td>
</tr>
</tr>
Line 185: Line 185:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>-</td><td>5</td><td>5</td>
<td>-</td><td>5</td><td>5</td>
</tr>
</tr>
Line 213: Line 213:
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O</td>
<td>2</td><td>-</td><td>-</td>
<td>2</td><td>-</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td><td>12</td><td>12</td>
+
<td>6μl</td><td>12μl</td><td>12μl</td>
</tr>
</tr>
</table>
</table>

Revision as of 19:57, 29 October 2008

>Home | Notebook

22 August 2008 <|> 24 August 2008

Contents

Meeting with UmeG

the ppt for our weekly meeting(japanese)

  1. Team:Input
  2. Team:Communication
  3. team:Output


Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep


--->Digestion test
  1. BBa_S03154(2007)
  2. BBa_S03154(2006)
  3. BBa_I9026(2007)
  4. BBa_I9026(2006)
  5. BBa_I9030(2007)
  6. BBa_I9030(2006)
  7. BBa_J04500(2007)


Sample No. 1- 671 - 71 - 7
DNA 3133
EcoRI --0.10.1
PstI --0.10.1
Buffer EcoRI --0.90.8
BSA --11
dH2O --55
TOTAL 3μl1μl10μl10μl


--->Gel Check
Chiba-0823-1.JPG
Sample No. 1~67
Sample DNA 31
Loading Dye 11
dH2O 24
TOTAL 6μl6μl
From left; Sanple No. 1~7
  1. BBa_S03154(2007)
  2. BBa_S03154(2006)
  3. BBa_I9026(2007)
  4. BBa_I9026(2006)
  5. BBa_I9030(2007)
  6. BBa_I9030(2006)
  7. BBa_J04500(2007)



Chiba-0823-2.JPG
Sample No. 8~21
Sample DNA 10
Loading Dye 2
TOTAL 12μl
From left;
(Single Digestion) Sanple No. 8~14
(Double Digestion) Sample No. 15~21



Digestion test
BBa_J04500(2007:old-sample)
Sample No. 123
Sample DNA 333
EcoRI -0.10.1
SpeI --0.1
EcoRI Buffer -0.90.8
BSA -11
dH2O -55
TOTAL 31010


--->Gel Check

Chiba-0823-3.JPG
Sample No. 123
Sample DNA 31010
Loading Dye 122
dH2O 2--
TOTAL 6μl12μl12μl
From left;
  1. BBa_J04500(2007) -> OK
  2. BBa_J04500(2007)Single Digestion -> OK
  3. BBa_J04500(2007)Double Digestion -> Bad

Team:Output

no work

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