Team:Chiba/Calendar-Home/23 August 2008

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22 August 2008 <|> 24 August 2008

Contents

1 Laboratory work

Laboratory work

Team:Input

no work

Team:Communication

(21/8)--->Mini prep

BBa_S03154(2007) [1]
BBa_S03154(2006) [2]
BBa_I9026(2007) [3]
BBa_I9026(2006) [4]
BBa_I9030(2007) [5]
BBa_I9030(2006) [6]

--->Digestion test

BBa_S03154(2007) [7]
BBa_S03154(2006) [8]
BBa_I9026(2007) [9]
BBa_I9026(2006) [10]
BBa_I9030(2007) [11]
BBa_I9030(2006) [12]
BBa_J04500(2007) [13]

Sample No	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21
BBa_I9030(2007) [14]	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-
BBa_I9030(2006) [15]	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-
BBa_S03154(2007) [16]	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-
BBa_S03154(2006) [17]	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-
BBa_I9026(2007) [18]	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-
BBa_I9026(2007) [19]	-	-	-	-	-	3	-	-	-	-	-	-	3	-	-	-	-	-	-	3	-
BBa_J04500(2007) [20]	-	-	-	-	-	-	1	-	-	-	-	-	-	3	-	-	-	-	-	-	3
EcoRⅠ	-	-	-	-	-	-	-	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1	0.1
PstⅠ	-	-	-	-	-	-	-	-	-	-	-	-	-	-	0.1	0.1	0.1	0.1	0.1	0.1	0.1
Buffer EcoRⅠ	-	-	-	-	-	-	-	0.9	0.9	0.9	0.9	0.9	0.9	0.9	0.8	0.8	0.8	0.8	0.8	0.8	0.8
BSA	-	-	-	-	-	-	-	1	1	1	1	1	1	1	1	1	1	1	1	1	1
dH2O	-	-	-	-	-	-	-	5	5	5	5	5	5	5	5	5	5	5	5	5	5
TOTAL	3	3	3	3	3	3	1	10	10	10	10	10	10	10	10	10	10	10	10	10	10

Sample No	1~6	7
Sample DNA	3	1
Loading Dye	1	1
dH2O	2	4
TOTAL	6	6

From left; Sanple No. 1~7

BBa_S03154(2007) [21]
BBa_S03154(2006) [22]
BBa_I9026(2007) [23]
BBa_I9026(2006) [24]
BBa_I9030(2007) [25]
BBa_I9030(2006) [26]
BBa_J04500(2007) [27]

Sample No	8~21
Sample DNA	10
Loading Dye	2
TOTAL	12

From left;

(Single Digestion) Sanple No. 8~14

BBa_S03154(2007) [28] -> OK
BBa_S03154(2006) [29] -> OK
BBa_I9026(2007) [30] -> OK
BBa_I9026(2006) [31] -> OK
BBa_I9030(2007) [32] -> OK
BBa_I9030(2006) [33] -> OK
BBa_J04500(2007) [34] -> OK

(Double Digestion) Sample No. 15~21

BBa_S03154(2007) [35] -> OK
BBa_S03154(2006) [36] -> OK
BBa_I9026(2007) [37] -> OK
BBa_I9026(2006) [38] -> OK
BBa_I9030(2007) [39] -> OK
BBa_I9030(2006) [40] -> OK
BBa_J04500(2007) [41] -> OK?

Digestion test

BBa_J04500(2007:old-sample)[42]

Sample No	1	2	3
Sample DNA	3	3	3
EcoRⅠ	-	0.1	0.1
SpeⅠ	-	-	0.1
EcoRⅠ Buffer	-	0.9	0.8
BSA	-	1	1
dH2O	-	5	5
TOTAL	3	10	10

--->Gel Check

Sample No	1	2	3
Sample DNA	3	10	10
Loading Dye	1	2	2
dH2O	2	-	-
TOTAL	6	12	12

From left;

BBa_J04500(2007) [43] -> OK
BBa_J04500(2007) [44]Single Digestion -> OK
BBa_J04500(2007) [45]Double Digestion -> Bad

Team:Output

no work

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