Team:Chiba/Calendar-Home/24 August 2008

From 2008.igem.org

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Latest revision as of 06:07, 30 October 2008

>Home | Notebook

23 August 2008 <|> 25 August 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

(22/8)--->Mini prep
  1. BBa_J04500(2007)
  2. BBa_J04500(2006)
  3. BBa_J13002(2007)
  4. BBa_J13002(2006)


--->Digestion test
  1. BBa_J04500(2007)
  2. BBa_J04500(2006)
  3. BBa_J13002(2007)
  4. BBa_J13002(2006)


Sample No. 5678
BBa_J04500(2007)(μL) 1---
BBa_J04500(2006)(μL) -1--
BBa_J13002(2007)(μL) --1-
BBa_J13002(2007)(μL) ---1
EcoRI(μL) 0.10.10.10.1
Buffer 4(μL) 0.90.90.90.9
dH2O(μL) 8888
TOTAL(μL) 10101010


--->Gel Check
Chiba-0824-1.JPG
Sample No. 1~8
Sample DNA(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
From left;
  1. BBa_J04500(2007) -> ?
  2. BBa_J04500(2006) -> OK
  3. BBa_J13002(2007) -> OK
  4. BBa_J13002(2006) -> OK
  5. BBa_J04500(2007) -> OK
  6. BBa_J04500(2006) -> OK
  7. BBa_J13002(2007) -> OK
  8. BBa_J13002(2006) -> None?
Chiba-0824-2.JPG
Sample No. 5~8
Sample DNA(μL) 9
Loading Dye(μL) 2
dH2O(μL) 1
TOTAL(μL) 12

From the left; (single digestuion)

5, BBa_J04500(2007) -> OK
6, BBa_J04500(2006) -> OK
7, BBa_J13002(2007) -> 3400bp:Bad, 2200bp:OK
8, BBa_J13002(2006) -> OK

Team:Output

Transformation

Digestion

Sample No. 123
DNA template(μL) 333
Buffer3(μL) 111
dH2O(μL) 666
EcoRI(μL) 0.50.50.5
TOTAL(μL) 101010


-->37°C 30min


Sample No. 1(afetr digestion)2(after digestion)3(afetr digestion)
DNA tamplate(μL) 101010
loading Dye(μL) 222
TOTAL(μL) 121212


Sample No. 123
DNA tamplate(μL) 222
loading Dye(μL) 111
dH2O(μL) 333
TOTAL(μL) 666

-->135V 30min