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Team:Chiba/Calendar-Home/25 August 2008
From 2008.igem.org
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<tr> | <tr> | ||
<td>DNA tamplate</td> | <td>DNA tamplate</td> | ||
| - | <td>1</td><td>1</td><td>1</td><td>1</td | + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 200: | Line 200: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>100μl</td><td>100μl</td><td>100μl</td><td>100μl</td><td>100μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 214: | Line 214: | ||
<tr> | <tr> | ||
<td>DNA tamplate</td> | <td>DNA tamplate</td> | ||
| - | <td>1</td><td>1</td><td>1</td><td>1</td | + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
| Line 226: | Line 226: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>6μl</td><td>6μl</td><td>6μl</td><td>6μl</td><td>6μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
| Line 278: | Line 278: | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | <td> | + | <td>100μl</td> |
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 15:13, 24 October 2008
24 August 2008 <|> 26 August 2008
Contents |
Laboratory work
Team:Input
The following glycerol Stock was made.
BBa_J22136(insert check済み)
プレートに生えたコロニーをpickして、試験管で2ml培養(LB-Amp,37℃,12h)
培養した試験管から180μl,LB-Ampを880μlとって、OD=0.495にした。
| Liquid culture(OD=0.495) | 0.67μl |
| 60%Glycerol | 0.33μl |
| 20%Glycerol Stock | 1.00ml |
Team:Communication
- (24/8)--->Digestion
- Double Digestion:BBa_J04500(2007)
- Double Digesiton:BBa_R0010(2007)
- Single Digestion:BBa_R0010(2007)
- BBa_R0010(2007)
| Sample No. | 1 | 2 | 3 | 4 |
| Sample DNA | 5 | 5 | 1 | 1 |
| EcoRⅠ | 0.5 | 0.5 | - | - |
| SpeⅠ | 0.5 | 0.5 | - | - |
| Buffer 1 | 1 | 1 | - | - |
| Buffer 3 | - | - | 1 | - |
| BSA | 1 | 1 | - | - |
| dH2O | 2 | 2 | 7.5 | - |
| TOTAL | 10 | 10 | 10 | 1 |
- --->Gel Check
|
|
--->Digestion
(Double Digestion)
- BBa_C0178
- BBa_C0170
- BBa_J04500(2007)
| Sample No. | 1 | 2 | 3 |
| Sample DNA | 33ng/μl×60μl | 33ng/μl×60μl | 100ng/μl×20μl |
| PstⅠ | 2 | 2 | 2 |
| XbaⅠ | 2 | 2 | - |
| SpeⅠ | - | - | 2 |
| Buffer 2 | - | - | 5 |
| Buffer 3 | 10 | 10 | - |
| BSA | 10 | 10 | 5 |
| dH2O | 16 | 16 | 16 |
| TOTAL | 100 | 100 | 52 |
Team:Output
- BBa_E2030①
- BBa_J33202②
- BL21③
- BBa_J52008④
- BBa_I712019⑤
| Sample No. | ① | ② | ③ | ④ | ⑤ |
| DNA tamplate | 1 | 1 | 1 | 1 | 1 |
| FW primer | 5(Venus_YFP_fwd) | 5(LacZ_fwd) | 5(LacZ_fwd) | 5(rLUC_fwd) | 5(fLuc_fwd) |
| VR primer | 5(VR) | 5(LacZ_rev) | 5(LacZ_rev) | 5(VR) | 5(VR) |
| dNTPmix | 10 | 10 | 10 | 10 | 10 |
| Thermo pol buffer | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 |
| dH2O | 68 | 68 | 68 | 68 | 68 |
| TOTAL | 100μl | 100μl | 100μl | 100μl | 100μl |
-->Gel Check
| Sample No. | ① | ② | ③ | ④ | ⑤ |
| DNA tamplate | 1 | 1 | 1 | 1 | 1 |
| loading Dye | 1 | 1 | 1 | 1 | 1 |
| dH2O | 4 | 4 | 4 | 4 | 4 |
| TOTAL | 6μl | 6μl | 6μl | 6μl | 6μl |
- Venus
- BBa_F2620
- Venus
- PUC19
- pGFPUV
- pGEX Venus YFP
- Membren Venus YFP
| Sample No. | ① |
| DNA tamplate | 1 |
| FW primer(Venus) | 5 |
| VR primer | 5 |
| dNTPmix | 10 |
| Thermo pol buffer | 10 |
| DNA pol(VENT) | 1 |
| dH2O | 68 |
| TOTAL | 100μl |
