Team:Chiba/Calendar-Home/25 August 2008
From 2008.igem.org
(Difference between revisions)
(→Team:Input) |
(→Team:Input) |
||
Line 10: | Line 10: | ||
<BR>Picked the colony and incubate with 2mL of LB-Ampicillin Medium for 12 hours at 37 degrees. | <BR>Picked the colony and incubate with 2mL of LB-Ampicillin Medium for 12 hours at 37 degrees. | ||
- | Took 180μl from culture,and mixed with 880& | + | Took 180μl from culture,and mixed with 880μlLB-Amp Medium.(OD=0.485) |
<table width="210" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | <table width="210" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> |
Revision as of 00:15, 30 October 2008
24 August 2008 <|> 26 August 2008
Contents |
Laboratory work
Team:Input
The following glycerol Stock was made.
BBa_J22136(insert check OK)
Picked the colony and incubate with 2mL of LB-Ampicillin Medium for 12 hours at 37 degrees.
Took 180μl from culture,and mixed with 880μlLB-Amp Medium.(OD=0.485)
Liquid culture(OD=0.495) | 0.67μl |
60%Glycerol | 0.33μl |
20%Glycerol Stock | 1.00ml |
Team:Communication
- (24/8)--->Digestion
- Double Digestion:BBa_J04500(2007)
- Double Digesiton:BBa_R0010(2007)
- Single Digestion:BBa_R0010(2007)
- BBa_R0010(2007)
Sample No. | 1 | 2 | 3 | 4 |
Sample DNA | 5 | 5 | 1 | 1 |
EcoRI | 0.5 | 0.5 | - | - |
SpeI | 0.5 | 0.5 | - | - |
Buffer 1 | 1 | 1 | - | - |
Buffer 3 | - | - | 1 | - |
BSA | 1 | 1 | - | - |
dH2O | 2 | 2 | 7.5 | - |
TOTAL | 10μl | 10μl | 10μl | 1μl |
- --->Gel Check
|
--->Digestion
(Double Digestion)
- BBa_C0178
- BBa_C0170
- BBa_J04500(2007)
Sample No. | 1 | 2 | 3 |
Sample DNA | 33ng/μl×60μl | 33ng/μl×60μl | 100ng/μl×20μl |
PstI | 2 | 2 | 2 |
XbaI | 2 | 2 | - |
SpeI | - | - | 2 |
Buffer 2 | - | - | 5 |
Buffer 3 | 10 | 10 | - |
BSA | 10 | 10 | 5 |
dH2O | 16 | 16 | 16 |
TOTAL | 100μl | 100μl | 52μl |
Team:Output
- BBa_E2030(1)
- BBa_J33202(2)
- BL21(3)
- BBa_J52008(4)
- BBa_I712019(5)
No. | 1 | 2 | 3 | 4 | 5 |
DNA tamplate | 1 | 1 | 1 | 1 | 1 |
FW primer | 5(Venus_YFP_fwd) | 5(LacZ_fwd) | 5(LacZ_fwd) | 5(rLUC_fwd) | 5(fLuc_fwd) |
VR primer | 5(VR) | 5(LacZ_rev) | 5(LacZ_rev) | 5(VR) | 5(VR) |
dNTPmix | 10 | 10 | 10 | 10 | 10 |
Thermo pol buffer | 10 | 10 | 10 | 10 | 10 |
DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 |
dH2O | 68 | 68 | 68 | 68 | 68 |
TOTAL | 100μl | 100μl | 100μl | 100μl | 100μl |
-->Gel Check
No. | 1 | 2 | 3 | 4 | 5 |
DNA tamplate | 1 | 1 | 1 | 1 | 1 |
loading Dye | 1 | 1 | 1 | 1 | 1 |
dH2O | 4 | 4 | 4 | 4 | 4 |
TOTAL | 6μl | 6μl | 6μl | 6μl | 6μl |
- BBa_E2030
- PUC19
- pGFPUV
- pGEX Venus YFP
- Membren Venus YFP
- BBa_J63001(1)
No. | 1 |
DNA tamplate | 1 |
FW primer(Venus) | 5 |
VR primer | 5 |
dNTPmix | 10 |
Thermo pol buffer | 10 |
DNA pol(VENT) | 1 |
dH2O | 68 |
TOTAL | 100μl |