Team:Chiba/Parts

From 2008.igem.org

(Difference between revisions)
(parts usages)
(parts usages)
Line 17: Line 17:
:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)]
:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0178 LasI(no LVA)]
-
日本語:シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。
+
日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。
English:
English:
Line 23: Line 23:
*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
*[http://partsregistry.org/Part:BBa_K084008 BBa_K084008]
:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]
:[http://partsregistry.org/Part:BBa_J04500 plac+rbs]+[http://partsregistry.org/Part:BBa_C0170 RhlI(no LVA)]
 +
*[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
 +
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]
-
日本語:シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
+
日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
English:
English:
Line 30: Line 32:
*[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
*[http://partsregistry.org/Part:BBa_K084009 BBa_K084009]
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9026 rbs+RhlI]
-
 
-
日本語:シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
 
-
 
-
English:
 
-
 
*[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]
*[http://partsregistry.org/Part:BBa_K084010 BBa_K084010]
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI]
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/Part:BBa_I9030 rbs+CinI]
 +
 +
日本語:IPTGにより、Rhizobium leguminosarum由来のC14HSL合成酵素,CinIを発現するデバイス。
 +
 +
English:
Line 44: Line 45:
*[http://partsregistry.org/Part:BBa_K084014 BBa_K084014]
*[http://partsregistry.org/Part:BBa_K084014 BBa_K084014]
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI]
:[http://partsregistry.org/Part:BBa_R0010 plac]+[http://partsregistry.org/wiki/index.php?title=Part:BBa_C0261 rbs+LuxI]
 +
 +
日本語:IPTGにより、Vibrio Fischeri由来の3OC6HSL合成酵素,LuxIを発現するデバイス。
 +
 +
English:
 +
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>
<table width="950" border="1" cellpadding="0" cellspacing="0" bordercolor="#000000" center>

Revision as of 11:30, 26 October 2008

Chiba-U.gif

Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements

We hope to submit these parts

Autoinducer synthase

parts usages

plac+rbs+LasI(no LVA)

日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。

English:

plac+rbs+RhlI(no LVA)
plac+rbs+RhlI

日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。

English:

plac+rbs+RhlI
plac+rbs+CinI

日本語:IPTGにより、Rhizobium leguminosarum由来のC14HSL合成酵素,CinIを発現するデバイス。

English:


plac+rbs+LuxI(no LVA)
plac+rbs+LuxI

日本語:IPTGにより、Vibrio Fischeri由来の3OC6HSL合成酵素,LuxIを発現するデバイス。

English:


Part Name Sub Parts Part Length(bp) Plasmid Vector Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
BBa_K084007 plac+rbs+LasI(no LVA) 835 pSB1AK3 3189 4024 Amp/Kan Glycerol stock in -80/miniprepped ok Chiba check.png
BBa_K084008 plac+rbs+RhlI(no LVA) 835 pSB1AK3 3189 4024 Amp/Kan Glycerol stock in -80/miniprepped ok Chiba check.png
BBa_K084009 plac+rbs+RhlI 893 pSB1A2 2079 2972 Amp Glycerol stock in -80/miniprepped ok Chiba check.png
BBa_K084010 plac+rbs+CinI 953 pSB1A2 2079 3032 Amp Glycerol stock in -80/miniprepped ok Chiba check.png
BBa_K084012 plac+rbs+LuxI(no LVA) 811 pSB1AK3 3189 4000 Amp/Kan Glycerol stock in -80/miniprepped Not yet Chiba check.png
BBa_K084014 plac+rbs+LuxI 869 pSB1A2 2079 2948 Amp Glycerol stock in -80/miniprepped Not yet Chiba check.png

Receiver protein

Part Name Sub Parts Part Length(bp) Plasmid Vector Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
BBa_K084004 ptet+rbs+RhlR 867 pSB1A2 2079 2946 Amp miniprepped ok Chiba check.png
BBa_K084004 ptet+rbs+RhlR 867 pSB3K3 2750 3617 Kan miniprepped Not yet Chiba check.png
BBa_K084005 ptet+rbs+LasR 861 pSB1A2 2079 2940 Amp miniprepped ok Chiba check.png
BBa_K084005 ptet+rbs+LasR 861 pSB3K3 2750 3611 Kan miniprepped Not yet Chiba check.png
BBa_K084006 ptet+rbs+CinR 867 pSB1A2 2079 2946 Amp miniprepped ok Chiba check.png
BBa_K084006 ptet+rbs+CinR 867 pSB3K3 2750 3617 Kan miniprepped Not yet Chiba check.png

Reporters

Part Name Sub Parts Part Length(bp) Plasmid Vector Part Length(bp) Part and vector length(bp) Resistance Status Sequenced Sent
BBa_K084002 rbs+venusYFP(yeast optimized) 767 miniprepped
BBa_K084003 ptet+LuxR+plux+venusYFP(yeast optimized) 1836 pSB1A2 2079 3915 Amp miniprepped OK Chiba check.png


Home The Team The Project Parts Submitted to the Registry Reference Notebook Acknowledgements