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Team:Chiba/Parts
From 2008.igem.org
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We hope to submit these parts
Autoinducer synthase
parts usages
日本語:IPTGにより、シュードモナス由来の3OC12HSL合成酵素,LasIを発現するデバイス。
English:
日本語:IPTGにより、シュードモナス由来のC4HSL,C6HSL合成酵素,RhlIを発現するデバイス。
English:
日本語:IPTGにより、Rhizobium leguminosarum由来の3-hydroxy-C14HSL合成酵素,CinIを発現するデバイス。
English:
日本語:IPTGにより、Vibrio Fischeri由来の3OC6HSL合成酵素,LuxIを発現するデバイス。
English:
Parts Status
| Part Name | Sub Parts | Part Length(bp) | Plasmid Vector | Part Length(bp) | Part and vector length(bp) | Resistance | Status | Sequenced | Sent | ||||||||
| BBa_K084007 | plac+rbs+LasI(no LVA) | 835 | pSB1AK3 | 3189 | 4024 | Amp/Kan | Glycerol stock in -80/miniprepped | ok |  | ||||||||
| BBa_K084008 | plac+rbs+RhlI(no LVA) | 835 | pSB1AK3 | 3189 | 4024 | Amp/Kan | Glycerol stock in -80/miniprepped | ok | | ||||||||
| BBa_K084009 | plac+rbs+RhlI | 893 | pSB1A2 | 2079 | 2972 | Amp | Glycerol stock in -80/miniprepped | ok | | ||||||||
| BBa_K084010 | plac+rbs+CinI | 953 | pSB1A2 | 2079 | 3032 | Amp | Glycerol stock in -80/miniprepped | ok | | ||||||||
| BBa_K084012 | plac+rbs+LuxI(no LVA) | 811 | pSB1AK3 | 3189 | 4000 | Amp/Kan | Glycerol stock in -80/miniprepped | Not yet | | ||||||||
| BBa_K084014 | plac+rbs+LuxI | 869 | pSB1A2 | 2079 | 2948 | Amp | Glycerol stock in -80/miniprepped | Not yet | |
Receiver protein
| Part Name | Sub Parts | Part Length(bp) | Plasmid Vector | Part Length(bp) | Part and vector length(bp) | Resistance | Status | Sequenced | Sent | ||||||||
| BBa_K084004 | ptet+rbs+RhlR | 867 | pSB1A2 | 2079 | 2946 | Amp | miniprepped | ok | | ||||||||
| BBa_K084004 | ptet+rbs+RhlR | 867 | pSB3K3 | 2750 | 3617 | Kan | miniprepped | Not yet | | ||||||||
| BBa_K084005 | ptet+rbs+LasR | 861 | pSB1A2 | 2079 | 2940 | Amp | miniprepped | ok | | ||||||||
| BBa_K084005 | ptet+rbs+LasR | 861 | pSB3K3 | 2750 | 3611 | Kan | miniprepped | Not yet | | ||||||||
| BBa_K084006 | ptet+rbs+CinR | 867 | pSB1A2 | 2079 | 2946 | Amp | miniprepped | ok | | ||||||||
| BBa_K084006 | ptet+rbs+CinR | 867 | pSB3K3 | 2750 | 3617 | Kan | miniprepped | Not yet | |
Reporters
| Part Name | Sub Parts | Part Length(bp) | Plasmid Vector | Part Length(bp) | Part and vector length(bp) | Resistance | Status | Sequenced | Sent | ||||||||
| BBa_K084002 | rbs+venusYFP(yeast optimized) | 767 | miniprepped | ||||||||||||||
| BBa_K084003 | ptet+LuxR+plux+venusYFP(yeast optimized) | 1836 | pSB1A2 | 2079 | 3915 | Amp | miniprepped | OK | |
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