Team:Chiba/Project

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== '''Integral Bacteria''' ==
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== '''Project Objection''' ==
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=== Project Summary ===
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Our project is to make ''E.coli'' process the amount of imput signal and read out the integrated value of the signals like ''E.coli'' performing integration.
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To do this, we construct two populations. ''E.coli'' A convert the amount of input linearly to expression of stable signal molecule which cannot metabolize or degradation. The stable molecule concentrainon is integrated value because the concentration does not go down. ''E.coli'' B contains another circuit to sense the concentration of the signal molecule and report. Thus E.coli B convert the integrated signal to PoPS.  The report circuit has some threshold to report the differnce of the concentration by expressing another fluorecent protein. To avoid mixing reporter proteins color or something, the reporter protein express only when the signal concentration over the threshold and induced
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Thus conbination of ''E.coli'' A and B, we can know how many input are integraed by expression
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of proteins.
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      Living things, environment and chemical samples are exposed to an input like as UV or radiation, which is "volatile" but whose effects to them accumulate in our body, environment and samples. We would like to know the total strength of exposure to the input, not only its existence.
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　　　To do this, we construct two populations. E.coli A synthesizes less-metabolic and stable molecules linearly to the strength of the input. The information of the total amount of the strength of the past input won't be deleted due to less-metabolicity and stability of these molecules. And they play a role as cell-cell communication signals. E.coli B senses the concentration of the signals and report how much strength have exposed to them. In this way, we can measure the total strength of exposure to UV, radiation, environmental pollutants and various kinds of substances as well just by changing the input sensor of E.coli A.
== Project Details==
== Project Details==

Revision as of 14:35, 1 August 2008

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Contents

Project Objection 

     Living things, environment and chemical samples are exposed to an input like as UV or radiation, which is "volatile" but whose effects to them accumulate in our body, environment and samples. We would like to know the total strength of exposure to the input, not only its existence.

　　　To do this, we construct two populations. E.coli A synthesizes less-metabolic and stable molecules linearly to the strength of the input. The information of the total amount of the strength of the past input won't be deleted due to less-metabolicity and stability of these molecules. And they play a role as cell-cell communication signals. E.coli B senses the concentration of the signals and report how much strength have exposed to them. In this way, we can measure the total strength of exposure to UV, radiation, environmental pollutants and various kinds of substances as well just by changing the input sensor of E.coli A.

Project Details

Part 2

The Experiments

Part 3

Results

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