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Team:Chiba/Sender experiments/Senders(XL10Gold) T9002(JW1908)
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design
Receiver
Method
- Transformed Senders into E.coli strains(JW1908) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37°C 12h
- Mixed them.
- Incubated at 37°C.
- Measured intensity of green fluorescence at regular time intervals.
Results
Reaction temparature:37°C
- センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,37°C,Receiver cells/Sender cells = 1.
Reaction temparature:30°C
- センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
E.coli strain,BBa_K084007:XL10Gold,BBa_T9002:JW1908,30°C,Receiver cells/Sender cells = 1.
Reaction temparature:25°C
センダーの培養液：500μL、レシーバの培養液：500μL
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.
E.coli strain,Senders:XL10Gold,BBa_T9002:JW1908,25°C,Receiver cells/Sender cells = 1.
Left:
- 蛍光強度が、30°C,37°Cに比べて極端に低く,Sendersの培養液を添加しなかった反応液と差がほとんどなかった.LuxI,LasI,RhlI,LuxR,GFPすべての発現量が減っていたためと考えた.
- LuxI,RhlIの培養液を,混ぜた反応液に比べて,LasIの培養液を混ぜた反応液は,最大蛍光強度が小さかった(約1/2).
Right:
- RhlIにLVAtagのついているものは,LVAtagのついていないものに比べて最大蛍光強度が小さかった.
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