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Team:Chiba/jk/α
From 2008.igem.org
(Difference between revisions)
| Line 4: | Line 4: | ||
== 2008.8.20== | == 2008.8.20== | ||
PCR | PCR | ||
| - | <BR>・BBa_J22136(2007) | + | :→'''[[Team:Chiba/protocol/PCR|PCR]]''' |
| - | <BR>・BBa_J22136(2008) | + | <BR>・BBa_J22136(2007)① |
| - | <BR>・BBa_J22141(2007) | + | <BR>・BBa_J22136(2008)② |
| - | <BR>・BBa_J22141(2008) | + | <BR>・BBa_J22141(2007)③ |
| - | <BR>・BBa_E0612(2008) | + | <BR>・BBa_J22141(2008)④ |
| + | <BR>・BBa_E0612(2008)⑤ | ||
| + | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| + | <tr> | ||
| + | <td width="257">Sample No</td> | ||
| + | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA tamplate</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>FW primer</td> | ||
| + | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>VR primer</td> | ||
| + | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dNTPmix</td> | ||
| + | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>thermo pol buffer</td> | ||
| + | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA pol(VENT)</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dH2O</td> | ||
| + | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>6</td><td>6</td><td>6</td><td>6</td><td>6</td> | ||
| + | </tr> | ||
| + | </table> | ||
<BR>→gel check | <BR>→gel check | ||
| - | <BR> | + | <BR>'''[[Team:Chiba/protocol/gel check|Gel Check]]''' |
| + | <table width="200" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
| + | <tr> | ||
| + | <td width="257">Sample No</td> | ||
| + | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>sample DNA</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>loading dye</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dH<sub>2</sub>O</td> | ||
| + | <td>4</td><td>4</td><td>4</td><td>4</td><td>4</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>total</td> | ||
| + | <td>6</td><td>6</td><td>6</td><td>6</td><td>6</td> | ||
| + | </tr> | ||
| + | |||
| + | </table> | ||
| + | →どれもバンド出ず。 | ||
| + | |||
| + | |||
| + | |||
<BR>trnsformation | <BR>trnsformation | ||
| Line 19: | Line 89: | ||
<BR>・BBa_J22136(2007) | <BR>・BBa_J22136(2007) | ||
<BR>・BBa_J22141(2007) | <BR>・BBa_J22141(2007) | ||
| + | |||
| + | →BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ) | ||
| + | |||
| + | == 2008.8.21 == | ||
| + | 8.20にtransformationしたもの | ||
| + | <BR>・BBa_R0051(2007) | ||
| + | <BR>・BBa_R0051(2006) | ||
| + | <BR>・BBa_J06650(2007) | ||
| + | <BR>・BBa_J06650(2006) | ||
| + | <BR>・BBa_J22136(2007) | ||
| + | <BR>・BBa_J22141(2007) | ||
| + | を２ml培養、12h。ただしコロニーが１個だけだったBBa_J22141は2ml培養の11h後にIPTGを20μl加え、 | ||
| + | さらに1h培養した。 | ||
| + | == 2008.8.22 == | ||
| + | mini prep | ||
| + | |||
Revision as of 10:55, 4 September 2008
入力班のぺーじ。
2008.8.20
PCR
- →PCR
・BBa_J22136(2007)①
・BBa_J22136(2008)②
・BBa_J22141(2007)③
・BBa_J22141(2008)④
・BBa_E0612(2008)⑤
| Sample No | ① | ② | ③ | ④ | ⑤ |
| DNA tamplate | 1 | 1 | 1 | 1 | >1 |
| FW primer | 5 | 5 | 5 | 5 | 5 |
| VR primer | 5 | 5 | 5 | 5 | 5 |
| dNTPmix | 10 | 10 | 10 | 10 | 10 |
| thermo pol buffer | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 |
| dH2O | 68 | 68 | 68 | 68 | 68 |
| TOTAL | 100 | 100 | 100 | 100 | 100 |
| TOTAL | 6 | 6 | 6 | 6 | 6 |
→gel check
Gel Check
| Sample No | ① | ② | ③ | ④ | ⑤ |
| sample DNA | 1 | 1 | 1 | 1 | >1 |
| loading dye | 1 | 1 | 1 | 1 | 1 |
| dH2O | 4 | 4 | 4 | 4 | 4 |
| total | 6 | 6 | 6 | 6 | 6 |
→どれもバンド出ず。
trnsformation
・BBa_R0051(2007)
・BBa_R0051(2006)
・BBa_J06650(2007)
・BBa_J06650(2006)
・BBa_J22136(2007)
・BBa_J22141(2007)
→BBa_J22141のみコロニーがほとんど生えていなかった。(１個のみ)
2008.8.21
8.20にtransformationしたもの
・BBa_R0051(2007)
・BBa_R0051(2006)
・BBa_J06650(2007)
・BBa_J06650(2006)
・BBa_J22136(2007)
・BBa_J22141(2007)
を２ml培養、12h。ただしコロニーが１個だけだったBBa_J22141は2ml培養の11h後にIPTGを20μl加え、
さらに1h培養した。
2008.8.22
mini prep
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