Team:Chiba/jk/γ

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'''17,August 2008'''
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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<br>PCR
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<br>BBa_E0430①
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<br>BBa_E0420②
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<br>BBa_J06702③
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<br>BBa_S01416④
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<br>BBa_I73003⑤
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<br>BBa_I73004⑥
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<br>BBa_I730026⑦
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<br>BBa_I763002⑧
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>FW primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>VR primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>dNTPmix</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>thermo pol buffer</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>DNA pol(VENT)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH2O</td>
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<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
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</tr>
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</table>
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'''20,August 2008'''
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>[[Team:Chiba/Internal|ノートに戻る]]
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<br>TRANSFORMATION
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<br>BBa_J52008(Luciferase Renilla)-->colony
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<br>BBa_I712019(Luciferase Fire fly)-->no colony
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<br>BBa_J332002(LacZ)-->colony
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<br>BBa_E2030(Venus YFP)-->not so good
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<br>YFP(P-GEX)-->colony
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<br>BBa_F2620-->colony
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<br>BBa_T9002-->colony
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'''21,August 2008'''
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== Out put team ==
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<br>TRANSFORMATION
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<br>BBa_J32007(Lux CDABE)-->no colony
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<br>BBa_B0034(RBS)-->colony
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'''22,August 2008'''
 
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<br>MINI PREP
 
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<br>BBa_J52008
 
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<br>BBa_F2620
 
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<br>BBa_T9002
 
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<br>
 
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'''24,August 2008'''
 
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<br>TRANSFORMATION
 
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<br>BBa_J63001(yeast optimized YFP)-->colony
 
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*'''2008,8,20(wed)'''
 
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Luciferase(J52008:2007,2008 I712019:2008),Lac Z(J33202:2007,2008),Venus(J32007:2007,2008),Craig Venus,T9002,F2620
 
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:結果:成功→Luciferase(J52008:2007),Lac Z(J33202:2007),Craig Venus,T9002,F2620
 
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::失敗→Luciferase(J52008:2008 J52008:2008),Lac Z(J33202:2008),Venus(J32007:2007,2008)
 
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*'''2008,8,21(thu)'''
 
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Lux CDABE(J32007:2007),RBS(B0034:2007)
 
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:結果:成功→RBS
 
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::失敗→Lux CDABE(二回行ったがどちらもコロニーができなかった)→三回目に成功
 
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*'''2008,8,22(sun)'''
 
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Venus YFP
 
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:結果
 
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===実験ノート===
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*[[Team:Chiba/jk/γ/week 1|week 1  (17,August~23,August)]]
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*[[Team:Chiba/jk/γ/week 2|week 2  (24,August~30,August)]]
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*[[Team:Chiba/jk/γ/week 3|week 3  (31,August~6,September)]]
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*[[Team:Chiba/jk/γ/week 4|week 4  (7,September~13,September)]]
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*[[Team:Chiba/jk/γ/week 5|week 5  (14,September~20,September)]]
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*[[Team:Chiba/jk/γ/week 6|week 6  (21,September~27,September)]]
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*[[Team:Chiba/jk/γ/week 7|week 7  (28,September~4,October)]]
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*[[Team:Chiba/jk/γ/week 7|week 7  (5,October~11,October)]]
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== [[Mini prep]] ==
 
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== [[AHL receiver]] ==
 
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== [[IPTG receiver]] ==
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===Time Responce Test===
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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<br>>[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
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<br>>[[Team:Chiba/jk/γ/trs|Time Responce Solid]]
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{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
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!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
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!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
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!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
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!align="center"|[[Team:Chiba/Internal|ノート]]
!align="center"|[[Team:Chiba/Internal|ノート]]
|}
|}

Latest revision as of 11:28, 14 December 2008

>ノートに戻る


Out put team

実験ノート


Time Responce Test


>Time Responce Liquid
>Time Responce Solid



ホーム メンバー紹介 プロジェクト紹介 作成したDNAパーツ ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk/%CE%B3"