Team:Chiba/jk/γ

From 2008.igem.org

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'''17,August 2008'''
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<html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Maiko/chiba.css&action=raw&ctype=text/css" type="text/css" /></html>
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<br>PCR
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<br>BBa_E0430①
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<br>BBa_E0420②
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<br>BBa_J06702③
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<br>BBa_S01416④
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<br>BBa_I73003⑤
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<br>BBa_I73004⑥
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<br>BBa_I730026⑦
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<br>BBa_I763002⑧
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>FW primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>VR primer</td>
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<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
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</tr>
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<tr>
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<td>dNTPmix</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>thermo pol buffer</td>
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<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>DNA pol(VENT)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH2O</td>
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<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
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</tr>
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</table>
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'''20,August 2008'''
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>[[Team:Chiba/Internal|ノートに戻る]]
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<br>TRANSFORMATION
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<br>BBa_J52008(Luciferase Renilla)-->colony
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<br>BBa_I712019(Luciferase Fire fly)-->no colony
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<br>BBa_J332002(LacZ)-->colony
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<br>BBa_E2030(Venus YFP)-->not so good
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<br>YFP(P-GEX)-->colony
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<br>BBa_F2620-->colony
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<br>BBa_T9002-->colony
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'''21,August 2008'''
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== Out put team ==
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<br>TRANSFORMATION
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<br>BBa_J32007(Lux CDABE)-->no colony
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<br>BBa_B0034(RBS)-->colony
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'''22,August 2008'''
 
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<br>MINI PREP
 
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<br>BBa_J52008(Luciferase)
 
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<br>BBa_F2620
 
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<br>BBa_T9002
 
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<br>BBa_J33202(LacZ)
 
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<br>PUC19
 
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<br>PGEX VENUS YFP(GST fusion)
 
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<br>
 
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<br>TRANSFORMATION
 
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<br>PGEX VENUS YFP(GST fusion)
 
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'''24,August 2008'''
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===実験ノート===
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<br>TRANSFORMATION
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<br>BBa_J63001(yeast optimized YFP)-->colony
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<br>Digestion
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<br>BBa_F2620(1)①
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<br>BBa_F2620(2)②
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<br>Menbren Venus YFP③
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>3</td><td>3</td><td>3</td>
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</tr>
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<tr>
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<td>Buffer3</td>
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<td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH2O</td>
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<td>6</td><td>6</td><td>6</td>
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</tr>
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<tr>
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<td>EcoRⅠ</td>
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<td>0.5</td><td>0.5</td><td>0.5</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>10</td><td>10</td><td>10</td>
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</tr>
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</table>
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<br>->37℃ 30min
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>①(afetr digestion)</td><td>②(after digestion)</td><td>③(afetr digestion)</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>10</td><td>10</td><td>10</td>
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</tr>
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<tr>
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<td>loading Dye</td>
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<td>2</td><td>2</td><td>2</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>12</td><td>12</td><td>12</td>
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</tr>
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</table>
+
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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-
<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>2</td><td>2</td><td>2</td>
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</tr>
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<tr>
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<td>loading Dye</td>
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<td>1</td><td>1</td><td>1</td>
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</tr>
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<tr>
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<td>dH2O</td>
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<td>3</td><td>3</td><td>3</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>6</td><td>6</td><td>6</td>
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</tr>
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</table>
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<br>->135V 30min
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-
'''25,August,2008'''
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*[[Team:Chiba/jk/γ/week 1|week 1 (17,August~23,August)]]
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<br>PCR
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*[[Team:Chiba/jk/γ/week 2|week 2  (24,August~30,August)]]
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<br>BBa_E2030①
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*[[Team:Chiba/jk/γ/week 3|week 3  (31,August~6,September)]]
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<br>BBa_J33202②
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*[[Team:Chiba/jk/γ/week 4|week 4  (7,September~13,September)]]
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<br>BL21③
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*[[Team:Chiba/jk/γ/week 5|week 5  (14,September~20,September)]]
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<br>BBa_J52008④
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*[[Team:Chiba/jk/γ/week 6|week 6  (21,September~27,September)]]
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<br>BBa_I712019⑤
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*[[Team:Chiba/jk/γ/week 7|week 7  (28,September~4,October)]]
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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*[[Team:Chiba/jk/γ/week 7|week 7  (5,October~11,October)]]
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<tr>
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<td width="257">Sample No</td>
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<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
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</tr>
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<tr>
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<td>DNA tamplate</td>
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<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
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</tr>
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<tr>
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<td>FW primer</td>
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<td>5(Venus_YFP_fwd)</td><td>5(LacZ_fwd)</td><td>5(LacZ_fwd)</td><td>5(rLUC_fwd)</td><td>5(fLuc_fwd)</td>
+
-
</tr>
+
-
<tr>
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-
<td>VR primer</td>
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<td>5(VR)</td><td>5(LacZ_rev)</td><td>5(LacZ_rev)</td><td>5(VR)</td><td>5(VR)</td>
+
-
</tr>
+
-
<tr>
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-
<td>dNTPmix</td>
+
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>thermo pol buffer</td>
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-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
-
</tr>
+
-
<tr>
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-
<td>DNA pol(VENT)</td>
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<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
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-
</tr>
+
-
<tr>
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-
<td>dH2O</td>
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-
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
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-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
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-
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
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-
</tr>
+
-
</table>
+
-
<br>->Gel
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-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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-
<tr>
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<td width="257">Sample No</td>
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-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA tamplate</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
+
-
</tr>
+
-
<tr>
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-
<td>loading Dye</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
+
-
</tr>
+
-
</table>
+
-
<br>MINI prep
 
-
<br>Venus
 
-
<br>BBa_F2620
 
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<br>TRANSFORMATRION
 
-
<br>Venus
 
-
<br>PUC19
 
-
<br>pGFPUV
 
-
<br>pGEX Venus YFP
 
-
<br>Membren Venus YFP
 
-
<br>PCR
 
-
<br>BBa_J63001①
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>FW primer(Venus)</td>
 
-
<td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>VR primer</td>
 
-
<td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dNTPmix</td>
 
-
<td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>thermo pol buffer</td>
 
-
<td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA pol(VENT)</td>
 
-
<td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>68</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>100</td>
 
-
</tr>
 
-
</table>
 
-
'''26,August,2008'''
 
-
<br>Gel check
 
-
<br>BBa_F2620
 
-
<br>BBa_F2620
 
-
<br>BBa_J63001
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>4</td><td>4</td><td>4</td><td>4</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td><td>6</td><td>6</td><td>6</td><td>6</td>
 
-
</tr>
 
-
</table>
 
-
<br>Digestion
 
-
<br>vector:BBa_F2620
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>BBa_F2620</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>100</td>
 
-
</tr>
 
-
<tr>
 
-
<td>SpeⅠ</td>
 
-
<td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA(x10)</td>
 
-
<td>13</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer2</td>
 
-
<td>13</td>
 
-
</tr>
 
-
<td>TOTAL</td>
 
-
<td>130</td>
 
-
</tr>
 
-
</table>
 
-
<br>insert:BBa_J52008,BBa_E2030
 
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<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>J52008</td><td>E2030</td>
 
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</tr>
 
-
<tr>
 
-
<td>DNA tamplate</td>
 
-
<td>30</td><td>30</td>
 
-
</tr>
 
-
<tr>
 
-
<td>XbaⅠ</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>DpnⅠ</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer3</td>
 
-
<td>4</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA(x10)</td>
 
-
<td>4</td><td>4</td>
 
-
</tr>
 
-
<td>TOTAL</td>
 
-
<td>40</td><td>40</td>
 
-
</tr>
 
-
</table>
 
-
<br>->37℃　3hour
 
-
<br>->SAP
 
-
<br>->37℃ 30min
 
-
<br>->65℃ 15min
 
-
<br>->Zymo Clean
 
-
<br>Ligation
 
-
<br>vector:BBa_F2620 insert:BBa_J52008①
 
-
<br>vector:BBa_F2620 insert:BBa_E2030②
 
-
<br>Negative control:BBa_F2620③
 
-
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>①</td><td>②</td><td>③</td>
 
-
</tr>
 
-
<tr>
 
-
<td>vector</td>
 
-
<td>5</td><td>5</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>inser</td>
 
-
<td>10</td><td>7.5</td><td>0</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Ligase Buffer</td>
 
-
<td>4</td><td>4</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Ligase</td>
 
-
<td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>0</td><td>2.5</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>20</td><td>20</td><td>20</td>
 
-
</tr>
 
-
</table>
 
-
<br>->RT 2hour
 
-
<br>->TRANSFORMATION XL10G
 
-
<br>->37℃ over night
 
-
<br>->colony PCR
 
-
<br>->MINI PREP
 
-
'''28,August,2008'''
+
===Time Responce Test===
-
<br>TIME RESPONCE
+
 +
<br>>[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
 +
<br>>[[Team:Chiba/jk/γ/trs|Time Responce Solid]]
-
 
+
{| style="color:white;" cellpadding="3" cellspacing="3" border="0" width="100%" align="center" class="menu" |
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-
 
+
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{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
+
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/j|ホーム]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Team/j|メンバー紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
!align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]]
-
!align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]]
+
!align="center"|[[Team:Chiba/Parts/j|作成したDNAパーツ]]
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!align="center"|[[Team:Chiba/Modeling/j|モデリング]]
+
!align="center"|[[Team:Chiba/Internal|ノート]]
!align="center"|[[Team:Chiba/Internal|ノート]]
|}
|}

Latest revision as of 11:28, 14 December 2008

>ノートに戻る


Out put team

実験ノート


Time Responce Test


>Time Responce Liquid
>Time Responce Solid



ホーム メンバー紹介 プロジェクト紹介 作成したDNAパーツ ノート
Retrieved from "http://2008.igem.org/Team:Chiba/jk/%CE%B3"