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Team:Chiba/jk/γ
From 2008.igem.org
(Difference between revisions)
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| - | + | '''17,August 2008''' | |
| - | + | <br>PCR | |
| - | + | <br>BBa_E0430① | |
| - | + | <br>BBa_E0420② | |
| - | + | <br>BBa_J06702③ | |
| - | + | <br>BBa_S01416④ | |
| - | + | <br>BBa_I73003⑤ | |
| - | + | <br>BBa_I73004⑥ | |
| - | + | <br>BBa_I730026⑦ | |
| - | + | <br>BBa_I763002⑧ | |
| - | + | <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000"> | |
| + | <tr> | ||
| + | <td width="257">Sample No</td> | ||
| + | <td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA tamplate</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>FW primer</td> | ||
| + | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>VR primer</td> | ||
| + | <td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dNTPmix</td> | ||
| + | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>thermo pol buffer</td> | ||
| + | <td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>DNA pol(VENT)</td> | ||
| + | <td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>dH2O</td> | ||
| + | <td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>TOTAL</td> | ||
| + | <td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td> | ||
| + | </tr> | ||
| + | </table> | ||
| + | |||
| + | '''20,August 2008''' | ||
| + | <br>TRANSFORMATION | ||
| + | <br>BBa_J52008(Luciferase Renilla)-->colony | ||
| + | <br>BBa_I712019(Luciferase Fire fly)-->no colony | ||
| + | <br>BBa_J332002(LacZ)-->colony | ||
| + | <br>BBa_E2030(Venus YFP)-->not so good | ||
| + | <br>YFP(P-GEX)-->colony | ||
| + | <br>BBa_F2620-->colony | ||
| + | <br>BBa_T9002-->colony | ||
| + | |||
| + | |||
| + | '''21,August 2008''' | ||
| + | <br>TRANSFORMATION | ||
| + | <br>BBa_J32007(Lux CDABE)-->no colony | ||
| + | <br>BBa_B0034(RBS)-->colony | ||
| + | |||
| + | '''22,August 2008''' | ||
| + | <br>MINI PREP | ||
| + | <br>BBa_J52008 | ||
| + | <br>BBa_F2620 | ||
| + | <br>BBa_T9002 | ||
| + | <br> | ||
| + | '''24,August 2008''' | ||
| + | <br>TRANSFORMATION | ||
| + | <br>BBa_J63001(yeast optimized YFP)-->colony | ||
== [[PCR]] == | == [[PCR]] == | ||
Revision as of 10:59, 9 September 2008
17,August 2008
PCR
BBa_E0430①
BBa_E0420②
BBa_J06702③
BBa_S01416④
BBa_I73003⑤
BBa_I73004⑥
BBa_I730026⑦
BBa_I763002⑧
| Sample No | ① | ② | ③ | ④ | ⑤ | ⑥ | ⑦ | ⑧ |
| DNA tamplate | 1 | 1 | 1 | 1 | >1 | 1 | 1 | 1 |
| FW primer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| VR primer | 5 | 5 | 5 | 5 | 5 | 5 | 5 | 5 |
| dNTPmix | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| thermo pol buffer | 10 | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| DNA pol(VENT) | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| dH2O | 68 | 68 | 68 | 68 | 68 | 68 | 68 | 68 |
| TOTAL | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
20,August 2008
TRANSFORMATION
BBa_J52008(Luciferase Renilla)-->colony
BBa_I712019(Luciferase Fire fly)-->no colony
BBa_J332002(LacZ)-->colony
BBa_E2030(Venus YFP)-->not so good
YFP(P-GEX)-->colony
BBa_F2620-->colony
BBa_T9002-->colony
21,August 2008
TRANSFORMATION
BBa_J32007(Lux CDABE)-->no colony
BBa_B0034(RBS)-->colony
22,August 2008
MINI PREP
BBa_J52008
BBa_F2620
BBa_T9002
24,August 2008
TRANSFORMATION
BBa_J63001(yeast optimized YFP)-->colony
Contents |
PCR
Transformation
Mini prep
AHL receiver
IPTG receiver
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