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Team:Chiba/jk/γ/Trl
From 2008.igem.org
(Difference between revisions)
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[[Team:Chiba/jk|実験ログ]] | [[Team:Chiba/jk|実験ログ]] | ||
| - | [[Team:Chiba/jk/γ|出力班]] | + | <br>[[Team:Chiba/jk/γ|出力班]] |
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*[[TEam:Chiba/jk/γ/TrI/0910|10,September,2008]] | *[[TEam:Chiba/jk/γ/TrI/0910|10,September,2008]] | ||
*[[TEam:Chiba/jk/γ/TrI/0914|14,September,2008]] | *[[TEam:Chiba/jk/γ/TrI/0914|14,September,2008]] | ||
| + | |||
| + | {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center" | ||
| + | !align="center"|[[Team:Chiba/j|ホーム]] | ||
| + | !align="center"|[[Team:Chiba/Team/j|メンバー紹介]] | ||
| + | !align="center"|[[Team:Chiba/Project/j|プロジェクト紹介]] | ||
| + | !align="center"|[[Team:Chiba/Parts/j|Parts Submitted to the Registry]] | ||
| + | !align="center"|[[Team:Chiba/Modeling/j|モデリング]] | ||
| + | !align="center"|[[Team:Chiba/Internal|ノート]] | ||
| + | |} | ||
Revision as of 03:01, 29 September 2008
Time Responce Liquid
装置&試薬
- 装置
- 46穴(Deep Well)
- しんとうき
- OD測定値
- 96穴Plate
- 蛍光リーダー
- 試薬
- IPTG(pLacの場合)
- AHL(pLuxの場合)
- X-gal(LacZの場合)
- M9
- PBS
プロトコル
- Pre培
- LB培養液(2ml)に固体培地から大腸菌をpickし３７℃で12時間培養(48穴Deep Well)
- 本培
- ODを計測し1/200に希釈しOD=0.01から1.0になるまで培養
- OD=1.0になったら生理食塩水でwash(2.0min 8,000rpm)x2
- M9またはPBSで培養し、IPTG,AHLを加え計測する。
- 0min,30min,60min,90min,120min,,,,,240minまで蛍光リーダー、OD値計測
- また、肉眼で確認
Result
| ホーム | メンバー紹介 | プロジェクト紹介 | Parts Submitted to the Registry | モデリング | ノート |
|---|
