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Team:ETH Zurich
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| - | Welcome to the ETH Zurich iGEM Wiki, the web site is under heavy reconstruction. | + | Welcome to the ETH Zurich iGEM Wiki, the web site is under heavy reconstruction so please be patient if most of the links are not available.<br> |
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| + | ===Project Abstract=== | ||
| + | ''''''Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome''''''<br> | ||
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| + | "This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome. | ||
| + | Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering. | ||
| + | We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is base on an iterative cycle of genome reduction and strain selection. | ||
| + | We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based selective condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection."<br> | ||
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| + | ==="Meet us" video=== | ||
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Revision as of 10:15, 12 October 2008
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Project Abstract'Make yourself simpler, stupid! Or how engineering a self-minimizing cell leads to the Minimal Genome' "This year's ETH Zurich project tackles a fundamental problem of synthetic biology: the minimal genome.
Exploring the minimal set of genes that is able to support life is not only a question of significant biological interest, it is also a crucial step towards the implementation of orthogonal functionalities into a rationally designed complex biological system. An organism carrying a minimal genome would provide a simple chassis for biological engineering.
We attempted to exploit the power of accelerated evolution for a genome reduction strategy. Our approach is base on an iterative cycle of genome reduction and strain selection.
We propose a novel method to randomly delete chromosomal DNA fragments by controlled expression of restriction enzymes and ligases in vivo. Furthermore we develop a chemostat-based selective condition to select for cells with a smaller genome size by constraining nucleotide availability. Computationally, we analyze the genome for the optimal restriction enzyme, and perform flux balance analysis on a genome scale model to predict growth of reduced genome strains. Finally, we simulate the restriction enzyme expression and the progression of selection." "Meet us" video
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