Team:Edinburgh/Modelling/Kinetic

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(New page: <div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div> ==Introduction== This section aims to outline the model formulation of the secretion pathway for cellulose degradat...)
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==Introduction==
==Introduction==
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This section aims to outline the model formulation of the secretion pathway  
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This section aims to outline the model of the cellulase secretion pathway for cellulose degradation as constructed by the University of  
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for cellulose degradation enzymes as constructed by the University of  
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Edinburgh 2008 iGEM team. The computational model includes two operons, 14 species, and 15 reactions, and  
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Edinburgh 2008 iGEM team.  
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focusses on the switching on of genes for cellulose degradation by CRP-cAMP.
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The pathway includes ...,
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The computational model includes two operons, 14 species, and 15 reactions, and  
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focusses on the switching on of genes for cellulose degradation by CRP.
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==Kinetic Modelling==
==Kinetic Modelling==
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A standard way of modelling (non-spatial) physical systems which has been  
A standard way of modelling (non-spatial) physical systems which has been  
successfully applied to simulate metabolic systems [Bakker et al., 1997] is the use of  
successfully applied to simulate metabolic systems [Bakker et al., 1997] is the use of  
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Ordinary Differential Equations (ODEs). The aim "of using a set of ODEs is to describe a biological phenomenon
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Ordinary Differential Equations (ODEs). The aim of using ODEs is to describe a biological phenomenon, so that the time dynamics of the system can be  analyzed or the steady state values of the variables involved in the model can be calculated. The effects of transcriptional regulation as well as of the changes in concentration of metabolites via enzymes are thus simulated.
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in order to analyze the time dynamics of the system or to calculate the steady state values of the variables
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involved in the model."and includes the effects of transcriptional regulation as well as of the changes in concentration of
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metabolites via enzymes.
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"The process of modelling tries to identify the variables and determine
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the relationships among them. The simulation (the execution of the model), allows to get
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results from the model. In case of differential equations models, the simulation
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refers simply to a numerical integration finding a solution to the set of equations.
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The goal of using a set of ODEs is to describe a biological phenomenon
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(like the nitrogen assimilation) in order to analyze the time dynamics
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of the system or to calculate the steady state values of the variables
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involved in the The goal of using a set of ODEs is to describe a biological phenomenon
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(like the nitrogen assimilation) in order to analyze the time dynamics
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of the system or to calculate the steady state values of the variables
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involved in the model."model."
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include the effects of gene regulation as well as the concentration
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changes of metabolites via enzymes.
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==The Biological System==
==The Biological System==
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The section we're most interested in modelling is the cellulase
 
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production leading to cell lysis pathway.
 
[[Image:Edinburgh-CellLysisModelling.jpg]]
[[Image:Edinburgh-CellLysisModelling.jpg]]
The system we sought to model is as follows:
The system we sought to model is as follows:
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Reduction in glucose concentration (due to?) leads to increased  
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Reduction in glucose concentration leads to increased adenylate cyclase activity. This results in more AMP being converted to cAMP.
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adenylate cyclase activity. This results in more AMP being converted to cAMP.
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cAMP binds to CRP. The resultant CRP-cAMP transcription factor activates the transcription of cellulase and cellubiase proteins (cenA, cex, and bglX) and comK via the cstA operator. ComK in turn binds to the comK operator, whereupon phiX174E, a lysis enzyme, is produced. Cell lysis releases the cellulases and bglX into the growth medium.
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cAMP binds to CRP. The resultant CRP-cAMP transcription factor binds
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to the *cstA* operator, causing the transcription of cellulase proteins  
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(cenA, cenB, cenC, and cex) and *comK*. ComK binds to the *comK*
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operator, whereupon *phiX174E*, a lysis enzyme, is produced. Cell lyses
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releases the cellulases into the growth medium.
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We chose to model the lysis pathway for the cellulose degradation
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==The Model==
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enzymes, since the activation of the associated genes may be the most
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interesting focus for modelling in our system of interest.
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cellobiose and beta-glucosidase... hydrolyses the cellobiose to glucose'
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Our modelling was done using [http://www.copasi.org/tiki-index.php COPASI].  
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(O'Neill et al, Gene 44:325-330).
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In the absence of experimental measurements, we have to fit likely
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[[Image:Edinburgh-ModelTable.jpg]]
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numbers...
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Our survey of the literature yielded some kinetic information for the
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genes in our system, in vivo, as well as in E. coli. 
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Our survey of the literature yielded some kinetic information for the genes in our system, in vivo, as well as in E. coli. Parameter values are currently being fit. 
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==The Model==
 
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[[Image:Edinburgh-ModelTable.jpg]]
 
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==ODEs==
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ODEs
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[[Image:Edinburgh-ModelODEs.jpg]]

Revision as of 03:25, 30 October 2008


Contents

Introduction

This section aims to outline the model of the cellulase secretion pathway for cellulose degradation as constructed by the University of Edinburgh 2008 iGEM team. The computational model includes two operons, 14 species, and 15 reactions, and focusses on the switching on of genes for cellulose degradation by CRP-cAMP.

Kinetic Modelling

Through mathematical modelling of cellular processes, we aim to reflect biological function in sets of equations supplied with parameter values that best fit observed reality (Drillon, G. 2008). If the outcomes of model-based simulations closely correspond to experimental results, this indicates that the underlying biological mechanism may be understood. Through the process of modelling, we may generate new hypotheses, as well as investigating doubts on previous suggestions, or corroborating them [Baralla, 2008]. Modelling in preparation and in response to wetlab work can save money and time by focussing on more likely hypotheses.

A standard way of modelling (non-spatial) physical systems which has been successfully applied to simulate metabolic systems [Bakker et al., 1997] is the use of Ordinary Differential Equations (ODEs). The aim of using ODEs is to describe a biological phenomenon, so that the time dynamics of the system can be analyzed or the steady state values of the variables involved in the model can be calculated. The effects of transcriptional regulation as well as of the changes in concentration of metabolites via enzymes are thus simulated.

The Biological System

Edinburgh-CellLysisModelling.jpg

The system we sought to model is as follows: Reduction in glucose concentration leads to increased adenylate cyclase activity. This results in more AMP being converted to cAMP. cAMP binds to CRP. The resultant CRP-cAMP transcription factor activates the transcription of cellulase and cellubiase proteins (cenA, cex, and bglX) and comK via the cstA operator. ComK in turn binds to the comK operator, whereupon phiX174E, a lysis enzyme, is produced. Cell lysis releases the cellulases and bglX into the growth medium.


The Model

Our modelling was done using COPASI.

Edinburgh-ModelTable.jpg

Our survey of the literature yielded some kinetic information for the genes in our system, in vivo, as well as in E. coli. Parameter values are currently being fit.


ODEs

Edinburgh-ModelODEs.jpg

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