Team:Edinburgh/Results

From 2008.igem.org

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<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div>
<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div>
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== Results: Current Status and Tests ==
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==Cellulolysis device==
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[[Image:Edinburgh Map1 Cellulolysis.jpg|150px]]
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===Cellulolysis device===
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* '''''cenA''''' (endoglucanase) and '''''cex''''' (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
* '''''cenA''''' (endoglucanase) and '''''cex''''' (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
* '''''bglX''''' (beta glucosidase): Biobrick made.
* '''''bglX''''' (beta glucosidase): Biobrick made.
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** [[Team:Edinbrugh/Results/PcstA-xylE|''P<sub>cstA</sub>'' promoter assay with ''xylE'' as repoter]]
** [[Team:Edinbrugh/Results/PcstA-xylE|''P<sub>cstA</sub>'' promoter assay with ''xylE'' as repoter]]
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=== Glycogenesis device ===
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== Glycogenesis device ==
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[[Image:Edinburgh Map2 Glycogen.jpg|150px]]
* '''''glgC''''' and '''''glgC16''''' (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful.
* '''''glgC''''' and '''''glgC16''''' (ADP-glucose pyrophosphorylase, responsible for rate limiting step in glycogenesis): Biobricks made and tested - successful.
** [[Team:Edinburgh/Results/Glycogen1|Glycogen Assay 1 (Qualitative)]]
** [[Team:Edinburgh/Results/Glycogen1|Glycogen Assay 1 (Qualitative)]]
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** [[Team:Edinburgh/Results/Glycogen3|Glycogen Assay 3 (Quantitative)]]
** [[Team:Edinburgh/Results/Glycogen3|Glycogen Assay 3 (Quantitative)]]
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=== Starch synthesis device ===
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== Starch synthesis device ==
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[[Image:Edinburgh Map3 Starch.jpg|150px]]
* '''''su1''''' and '''''iso2''''' (isoamylases): Work in progress - to be continued in an undergraduate honours project.
* '''''su1''''' and '''''iso2''''' (isoamylases): Work in progress - to be continued in an undergraduate honours project.
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===Lycopene generator ===
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==Lycopene generator ==
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[[Image:Edinburgh Map4 BCarotene.jpg|150px]]
* '''''dxs+crtE+crtB+crtI+crtY''''': Work in progress - encountered problem at final step.
* '''''dxs+crtE+crtB+crtI+crtY''''': Work in progress - encountered problem at final step.
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===Limonene synthase device===
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==Limonene synthase device==
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[[Image:Edinburgh Map5 Limonene.jpg|150px]]
* '''''dxs+LIMS+appY''''': Biobrick made - still being tested (last test failed to detect limonene synthesis).
* '''''dxs+LIMS+appY''''': Biobrick made - still being tested (last test failed to detect limonene synthesis).

Revision as of 20:52, 27 October 2008

Contents

Cellulolysis device

Edinburgh Map1 Cellulolysis.jpg

  • cenA (endoglucanase) and cex (exoglucanase): Biobricks made, but encountered problems when adding ribosome binding site.
  • bglX (beta glucosidase): Biobrick made.
  • PcstA (promoter): Biobrick made and tested - successful.

Glycogenesis device

Edinburgh Map2 Glycogen.jpg

Starch synthesis device

Edinburgh Map3 Starch.jpg

  • su1 and iso2 (isoamylases): Work in progress - to be continued in an undergraduate honours project.

Lycopene generator

Edinburgh Map4 BCarotene.jpg

  • dxs+crtE+crtB+crtI+crtY: Work in progress - encountered problem at final step.

Limonene synthase device

Edinburgh Map5 Limonene.jpg

  • dxs+LIMS+appY: Biobrick made - still being tested (last test failed to detect limonene synthesis).