Team:Guelph/Modeling

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!style="font-size:140%; text-align:center; background-color:#ffff00; border-width:0px; padding:3px;"|[[Team:Guelph|<font color="#cd0000">Home</font>]]
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!style="font-size:140%;text-align:center; background-color:#ffff00; border-width:0px; padding:3px;"|[[Team:Guelph/Team|<font color="#cd0000">The Team</font>]]
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!style="font-size:140%;text-align:center; background-color:#ffff00; border-width:0px; padding:3px;"|[[Team:Guelph/Project|<font color="#cd0000">The Project</font>]]
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== '''Making projections based on measurements on endosymbiont and endophyte numbers within a functioning biological system using GFP''' ==
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'''Making projections based on measurements on endosymbiont and endophyte numbers within a functioning biological system using GFP'''  
   
   
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We plan to do wet lab modelling. By tagging our microbes with simple GFP constructs and taking in vivo measurments of fluorescence levels, we hope to be able to estimate nutrient or RNAi levels produced within eukaryotic hosts. More to come as this develops.
 
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===Note===
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We plan to do wet lab modelling. By tagging our microbes with simple GFP constructs and counting colony forming units per set sample fresh weight, we hope to be able to observe microbial survival with our construct and predict how much beta carotene or RNAi might be produced and released.
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In corn, we are doing this using a corn endophyte called Klebsiella pneumonii. Plants were either injected with 5 ul of bacterial suspension or dipped in it at an early stage. Two weeks later (a month for the dipped plants) 500 mg of tissue were harvested, ground in sterilized mortars, resuspended in 500 ul of sodium phosphate buffer, and 50 ul of the dilution was spread on half of a kanamycin LB plate.  The results are depicted graphically below.
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If you choose to include a '''Modeling''' page, please write about your modeling adventures here.  This is not necessary but it may be a nice list to include.
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[[Image:KP342 GFP assay in corn.jpg|700px]]

Latest revision as of 05:32, 27 September 2008


Home The Team The Project Parts Notebook Results Links


Making projections based on measurements on endosymbiont and endophyte numbers within a functioning biological system using GFP


We plan to do wet lab modelling. By tagging our microbes with simple GFP constructs and counting colony forming units per set sample fresh weight, we hope to be able to observe microbial survival with our construct and predict how much beta carotene or RNAi might be produced and released.

In corn, we are doing this using a corn endophyte called Klebsiella pneumonii. Plants were either injected with 5 ul of bacterial suspension or dipped in it at an early stage. Two weeks later (a month for the dipped plants) 500 mg of tissue were harvested, ground in sterilized mortars, resuspended in 500 ul of sodium phosphate buffer, and 50 ul of the dilution was spread on half of a kanamycin LB plate. The results are depicted graphically below.

KP342 GFP assay in corn.jpg

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