Team:Harvard/Dailybook/Week5/Widgetry

From 2008.igem.org

(Difference between revisions)
(Wednesday: July 23, 2008)
(Thursday: July 24, 2008)
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=Thursday: July 24, 2008=
=Thursday: July 24, 2008=
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* mtrB 1 day vs. 2 day experiment
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** 6 chambers
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*** 3 chambers with 1-day old mtrB
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*** 3 chambers with 2-day old mtrB
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*** Injected lactate after the initial current spike came down
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=Friday: July 25, 2008=
=Friday: July 25, 2008=
[[Image:mtrb_1dv2d.jpg | 600px]]
[[Image:mtrb_1dv2d.jpg | 600px]]

Revision as of 21:52, 28 October 2008

Contents

Goals for the Week

  • Last week, had experiment with no current at all
    • Hypothesized that cells were dead?
    • Run test with 1 day vs. 2 day old Shewie to determine if there is an effect

Monday: July 21, 2008

  • Lab meeting
  • Cleaned chambers

Tuesday: July 22, 2008

  • started mtrB and wt cultures
    • mtrB culture to be used as 2-day culture on Thursday
  • troubleshoot LabVIEW
    • Resistances were measured to be different as expected in the past experiment
    • Some as low as 160 ohms
    • Perhaps the resistance of the chambers are less than we expected?

Wednesday: July 23, 2008

  • Started another mtrB culture
    • To be used as 1 day culture in experiment on Thursday
  • LabVIEW
    • Determined that resistance should be measured before connecting to the fuel cells
    • Resistance of chambers is the same as what we measured initially

Thursday: July 24, 2008

  • mtrB 1 day vs. 2 day experiment
    • 6 chambers
      • 3 chambers with 1-day old mtrB
      • 3 chambers with 2-day old mtrB
      • Injected lactate after the initial current spike came down

Friday: July 25, 2008

Mtrb 1dv2d.jpg

mtrB: Overnight versus 2 Day Liquid Cultures


What we did:

-Ran 1-day old mtrB versus 2-day old mtrB


Results:

-The shapes of the curves were all very similar.

-Overnight cultures had slightly higher current production than 2-day old cultures.