Team:Harvard/Dailybook/Week9/Widgetry

From 2008.igem.org

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(Friday: August 21, 2008)
(Friday: August 21, 2008)
 
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=Monday: August 17, 2008=
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=Baseline Experiment for Chemical/Heat Inducible Systems=
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[[Image:bline1.jpg | 600px]]
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=Tuesday: August 18, 2008=
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=Wednesday: August 19, 2008=
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=Thursday: August 20, 2008=
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=Friday: August 21, 2008=
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[[Image:Baseline1.jpg | 600px]]
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'''Baseline Chemical and Heat Test'''
'''Baseline Chemical and Heat Test'''
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What we did:
What we did:
-Tested wt and mtrB strains of Shewie with adding IPTG, Tet, and heating to 40C
-Tested wt and mtrB strains of Shewie with adding IPTG, Tet, and heating to 40C
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Results:
Results:
-Current production was very low for all chambers
-Current production was very low for all chambers
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:-Less than 3 microAmps even for wt Shewie which is nearly a hundred-fold lower than what we normally found
:-Less than 3 microAmps even for wt Shewie which is nearly a hundred-fold lower than what we normally found
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-Chambers were not anaerobic
-Chambers were not anaerobic
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-Need to redo this test
-Need to redo this test

Latest revision as of 01:39, 30 October 2008

Baseline Experiment for Chemical/Heat Inducible Systems

Bline1.jpg

Baseline Chemical and Heat Test


What we did:

-Tested wt and mtrB strains of Shewie with adding IPTG, Tet, and heating to 40C


Results:

-Current production was very low for all chambers

-Less than 3 microAmps even for wt Shewie which is nearly a hundred-fold lower than what we normally found

-Chambers were not anaerobic

-Need to redo this test