Team:Harvard/Hardware/MFCProcedure

From 2008.igem.org

(Difference between revisions)
(Running an MFC Experiment from Start to Finish)
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=Running an MFC Experiment=
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This page is intended as a comprehensive guide to completing a microbial fuel cell experiment and running an experiment from start to finish.
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==Creating a Testing Environment==
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Begin 1-2 weeks prior to experiment
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===Constructing Fuel Cell Components===
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 +
===Setup of Digital Multimeter===
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===Controlling the DMM with LabView™===
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==Experiment Preparation==
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Begin 1 day prior to experiment
 +
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===Assembling Chambers===
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===Solutions Prep===
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Chamber media
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* 5.844 g/L 100mM NaCl
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* 15.1185 g/L 50mM PIPES (hydrogen)
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''7.0 pH''
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Phosphate buffer
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* 2.918 g/L Monosodium phosphate, monohydrate
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* 4.095 g/L Disodium phosphate, anhydrous 
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* 5.844 g/L 100mM NaCl
 +
''7.0 pH''
 +
 +
===Gas Tubing Assembly===
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===Growing Strains===
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==Runtime==
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Begin 2 hours prior to experiment
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===Bacteria===
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===Fuel Cells===
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===Injections/Variables===
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==Clean Up==
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=Running an MFC Experiment=
=Running an MFC Experiment=
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==Clean Up==
==Clean Up==
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Revision as of 22:15, 28 October 2008

Running an MFC Experiment

This page is intended as a comprehensive guide to completing a microbial fuel cell experiment and running an experiment from start to finish.

Creating a Testing Environment

Begin 1-2 weeks prior to experiment

Constructing Fuel Cell Components

Setup of Digital Multimeter

Controlling the DMM with LabView™

Experiment Preparation

Begin 1 day prior to experiment

Assembling Chambers

Solutions Prep

Chamber media

  • 5.844 g/L 100mM NaCl
  • 15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

  • 2.918 g/L Monosodium phosphate, monohydrate
  • 4.095 g/L Disodium phosphate, anhydrous
  • 5.844 g/L 100mM NaCl

7.0 pH

Gas Tubing Assembly

Growing Strains

Runtime

Begin 2 hours prior to experiment

Bacteria

Fuel Cells

Injections/Variables

Clean Up



Running an MFC Experiment

This page is intended as a comprehensive guide to completing a microbial fuel cell experiment and running an experiment from start to finish.

Creating a Testing Environment

Begin 1-2 weeks prior to experiment

Constructing Fuel Cell Components

Setup of Digital Multimeter

Controlling the DMM with LabView™

Experiment Preparation

Begin 1 day prior to experiment

Assembling Chambers

Solutions Prep

Chamber media

  • 5.844 g/L 100mM NaCl
  • 15.1185 g/L 50mM PIPES (hydrogen)

7.0 pH

Phosphate buffer

  • 2.918 g/L Monosodium phosphate, monohydrate
  • 4.095 g/L Disodium phosphate, anhydrous
  • 5.844 g/L 100mM NaCl

7.0 pH

Gas Tubing Assembly

Growing Strains

Runtime

Begin 2 hours prior to experiment

Bacteria

Fuel Cells

Injections/Variables

Clean Up