From 2008.igem.org
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| - | =Running an MFC Experiment=
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| - | This page is intended as a comprehensive guide to completing a microbial fuel cell experiment and running an experiment from start to finish.
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| - | ==Creating a Testing Environment==
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| - | Begin 1-2 weeks prior to experiment
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| - | ===Constructing Fuel Cell Components===
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| - | ===Setup of Digital Multimeter===
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| - | ===Controlling the DMM with LabView™===
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| - | ==Experiment Preparation==
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| - | Begin 1 day prior to experiment
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| - | ===Assembling Chambers===
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| - | ===Solutions Prep===
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| - | Chamber media
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| - | * 5.844 g/L 100mM NaCl
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| - | * 15.1185 g/L 50mM PIPES (hydrogen)
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| - | ''7.0 pH''
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| - | Phosphate buffer
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| - | * 2.918 g/L Monosodium phosphate, monohydrate
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| - | * 4.095 g/L Disodium phosphate, anhydrous
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| - | * 5.844 g/L 100mM NaCl
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| - | ''7.0 pH''
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| - | ===Gas Tubing Assembly===
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| - | ===Growing Strains===
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| - | ==Runtime==
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| - | Begin 2 hours prior to experiment
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| - | ===Bacteria===
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| - | ===Fuel Cells===
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| - | ===Injections/Variables===
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| - | ==Clean Up==
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| | __NOTOC__ | | __NOTOC__ |
| | <html> | | <html> |
Revision as of 22:15, 28 October 2008
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Running an MFC Experiment
This page is intended as a comprehensive guide to completing a microbial fuel cell experiment and running an experiment from start to finish.
Creating a Testing Environment
Begin 1-2 weeks prior to experiment
Constructing Fuel Cell Components
Setup of Digital Multimeter
Controlling the DMM with LabView™
Experiment Preparation
Begin 1 day prior to experiment
Assembling Chambers
Solutions Prep
Chamber media
- 5.844 g/L 100mM NaCl
- 15.1185 g/L 50mM PIPES (hydrogen)
7.0 pH
Phosphate buffer
- 2.918 g/L Monosodium phosphate, monohydrate
- 4.095 g/L Disodium phosphate, anhydrous
- 5.844 g/L 100mM NaCl
7.0 pH
Gas Tubing Assembly
Growing Strains
Runtime
Begin 2 hours prior to experiment
Bacteria
Fuel Cells
Injections/Variables
Clean Up
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