Team:Harvard/Parts

From 2008.igem.org

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<a href="https://2008.igem.org/Team:Harvard/Team"><img src="https://static.igem.org/mediawiki/2008/3/3e/HTeam1.jpg"></a>
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This is a template page. READ THESE INSTRUCTIONS.
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<a href="https://2008.igem.org/Team:Harvard/Project"><img src="https://static.igem.org/mediawiki/2008/3/3c/HProject1.jpg"></a>
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<a href="https://2008.igem.org/Team:Harvard/Parts"><img src="https://static.igem.org/mediawiki/2008/0/0a/HRegistry1.jpg"></a>
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<a href="https://2008.igem.org/Team:Harvard/Modeling"><img src="https://static.igem.org/mediawiki/2008/a/a2/HModelling1.jpg"></a>
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<a href="https://2008.igem.org/Team:Harvard/Notebook"><img src="https://static.igem.org/mediawiki/2008/b/ba/HNotebook.jpg"></a>
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, and a lab notebook. PLEASE keep all of your pages within your Team:Example namespace. 
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''Tell us more about your project.  Give us backgroundUse this is the abstract of your projectBe descriptive but concise (1-2 paragraphs)''
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The 2008 Harvard iGEM project is based on the ability to control and measure the electric current production in Shewanella oneidensis MR-1Multiple genes in Shewanella have been identified as playing a role in transferring electrons to solid substrates, thus generating an easily detectible electric currentOur goal is to be able to detect differences in current output by the activation and/or deactivation of these genes in ShewanellaTo do this, we are using mutant strains of Shewanella which have one or more of these important electron transfer genes knocked out and are reintroducing the genes on an inducible plasmid. The electric current produced will be detected by a multimeter, and read by a computer, creating a computer-biology interface.
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|align="center"|[[Team:Harvard | Team Example 2]]
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We are developing two inducible systems for electrical current production in Shewanella.  The first is a chemically inducible system, where LacI or TetR repression of the current-production gene expression can be alleviated by the addition of IPTG or anhydrotetracycline, respectively.  Our second approach is a light-inducible system based on the 2005 UT Austin biological camera.  We are also considering a third approach using cI lambda which would be dependent on temperature.
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Using one or both of these systems, we hope to be able to interact with engineered Shewanella in multiple anaerobic microbial fuel cells which we have built. Communication between the fuel cells and a computer then has the potential to be programmable into a game of sorts, such as Tic-Tac-Toe, in which the level of current production induced by a chemical or light input is understood by the computer as a "turn." This rudimentary game is just one of many possibilities for an inducible interaction between computers and bacteria.
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!align="center"|[[Team:Harvard|Home]]
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!align="center"|[[Team:Harvard/Team|The Team]]
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!align="center"|[[Team:Harvard/Project|The Project]]
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!align="center"|[[Team:Harvard/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Harvard/Modeling|Modeling]]
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!align="center"|[[Team:Harvard/Notebook|Notebook]]
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(''Or you can choose different headings.  But you must have a team page, a project page, and a notebook page.'')
 
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===Note===
 
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If you choose to include a '''Parts Submitted to the Registry''' page, please list your parts here.  This is not necessary but it may be a nice list to keep track of.
 

Revision as of 17:37, 26 October 2008



The 2008 Harvard iGEM project is based on the ability to control and measure the electric current production in Shewanella oneidensis MR-1. Multiple genes in Shewanella have been identified as playing a role in transferring electrons to solid substrates, thus generating an easily detectible electric current. Our goal is to be able to detect differences in current output by the activation and/or deactivation of these genes in Shewanella. To do this, we are using mutant strains of Shewanella which have one or more of these important electron transfer genes knocked out and are reintroducing the genes on an inducible plasmid. The electric current produced will be detected by a multimeter, and read by a computer, creating a computer-biology interface.

We are developing two inducible systems for electrical current production in Shewanella. The first is a chemically inducible system, where LacI or TetR repression of the current-production gene expression can be alleviated by the addition of IPTG or anhydrotetracycline, respectively. Our second approach is a light-inducible system based on the 2005 UT Austin biological camera. We are also considering a third approach using cI lambda which would be dependent on temperature.

Using one or both of these systems, we hope to be able to interact with engineered Shewanella in multiple anaerobic microbial fuel cells which we have built. Communication between the fuel cells and a computer then has the potential to be programmable into a game of sorts, such as Tic-Tac-Toe, in which the level of current production induced by a chemical or light input is understood by the computer as a "turn." This rudimentary game is just one of many possibilities for an inducible interaction between computers and bacteria.