Team:Harvard/Plasmids
From 2008.igem.org
(Difference between revisions)
Joyy (Talk | contribs)
(New page: == Vectors == *pACYC-Duet vector map *[http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3] ==Parts== *[http://spreadsheets.google.com/ccc?key=pwvx...)
Newer edit →
(New page: == Vectors == *pACYC-Duet vector map *[http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3] ==Parts== *[http://spreadsheets.google.com/ccc?key=pwvx...)
Newer edit →
Revision as of 19:44, 26 October 2008
Vectors
- pACYC-Duet vector map
- [http://partsregistry.org/wiki/index.php?title=Part:pSB3K3 pSB3K3]
Parts
- [http://spreadsheets.google.com/ccc?key=pwvxAM8xwIq37aJ-qKhmUag&hl=en Google Spreadsheet]
Name | Registry Name | Description | Origin | Size | Marker | Transformed? | Miniprepped? | Culture in Glycerol Stock? | Sequenced? | |
P1 | [http://partsregistry.org/Part:BBa_I20259 J23113/I20259] | Low promoter GFP tester | p15A pMR101-derived | 919 | Kan | in S1 and TOP10 | Yes for both strains | Yes for both strains. | ||
P2 | [http://partsregistry.org/Part:BBa_I20269 J23150/I20269] | Medium promoter GFP tester | p15A pMR101-derived | 919 | Kan | in TOP10, not S1 | ||||
P3 | [http://partsregistry.org/Part:BBa_I20270 J23151/I20270] | High promoter GFP tester | p15A pMR101-derived | 919 | Kan | in TOP10 and S1 | Yes for both strains. | Yes for both strains. | ||
P4 | [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pACYC duet] | Low-Medium copy vector | p15a | 4008 | Cm | Yes | in S1 and TOP10 | |||
P5 | [http://partsregistry.org/Part:pSB3K3 pSB3K3] | Low-Medium copy vector (w/ death gene) | p15a | 2750 | Kan | |||||
P6 | [http://partsregistry.org/Part:BBa-E1010 BBa-E1010] | RFP only (w/ death gene) | pMB1 | 681 | Kan | |||||
P7 | [http://partsregistry.org/Part:pSB1A2 pSB1A2] | GFP only | pMB1 | 2079 | Amp | |||||
P8 | [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] | RFP with LacI promoter | pMB1 | 1069 | Amp | |||||
P9 | [http://partsregistry.org/Part:BBa_J04430 BBa_J04430] | GFP with LacI promoter | pMB1 | 1083 | Amp | |||||
P10 | [http://partsregistry.org/Part:BBa_I715038 BBa_I715038] | T7 Polymerase with LacI promoter | pMB1 | 2878 | Amp | |||||
P11 | [http://partsregistry.org/Part:BBa_M30109 BBa_M30109] | Light responsive system, dual regulation | pUC19-derived pMB1 | 4333 | Amp/Cm | |||||
P12 | [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pETDuet-1] | Low copy plasmid | pBR322-derived ColE1 | 5420 | Amp | |||||
P13 | [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCDFDuet-1] | Low copy plasmid | CloDF13 | 3781 | Sm | Yes | in S1 | |||
P14 | [http://www.merckbiosciences.co.uk/html/NVG/duettable.html pCOLADuet-1] | Low copy plasmid | ColA | 3719 | Kan | |||||
P15 | [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] | GFP with Tet promoter | ColE1 | 937 | Amp | |||||
P16 | [http://partsregistry.org/Part:BBa_R0040 BBa_R0040] | Tet repressible promoter | pMB1 | 54 | Amp | |||||
P17 | [http://partsregistry.org/Part:BBa_Q04400 BBa_Q04400] | Inverter (TetR w/o promoter and Tet promoter) | OriS, P1 lytic, F1 | 902 | Kan | |||||
P18 | [http://partsregistry.org/Part:BBa_R0051 BBa_R0051] | lambda promoter (cI regulated) | pMB1 | 49 | Amp | |||||
P19 | [http://partsregistry.org/Part:BBa_I763007 BBa_I763007] | RFP with lambda promoter | pMB1 | 918 | Amp | |||||
P20 | [http://partsregistry.org/Part:BBa_J04431 BBa_J04431] | GFP (LVA tagged) with Lac promoter | pMB1 | 1122 | Amp, Kan | |||||
P21 | A1 (pACYC) | pACYC (P4) w/ biobricks | ??? | ??? | ??? | Yes | in S1 and TOP10 | |||
P22 | [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] | ompR controlled promoter for ompC (long) | pMB1 | 108 | Amp | |||||
P23 | [http://partsregistry.org/Part:BBa_R0083 BBa_R0083] | ompR controlled promoter for ompC (short) | pMB1 | 78 | Amp | |||||
P24 | [http://partsregistry.org/Part:BBa_R0084 BBa_R0084] | ompR controlled promoter for ompF | pMB1 | 108 | Amp | |||||
P25 | [http://partsregistry.org/Part:BBa_J58102 BBa_J58102] | Promoter activated by OmpR-P with the reporter GFP | pUC19-derived pMB1 | 991 | Amp | |||||
P26 | [http://partsregistry.org/Part:BBa_J58103 BBa_J58103] | Mutated promoter activated by OmpR-P with the reporter GFP | pUC19-derived pMB1 | 961 | Amp | |||||
P27 | pZS4int2 | repressor plasmid | pSC101 | Sm | Yes | |||||
P28 | pZE21 | GFP | ColE1 | Kan | Yes | |||||
P29 | pZS*2R | Venus (mutant YFP) | pSC101* | Kan | Yes | |||||
P30 | pZA32 | YFP | p15A | Cm | Yes | |||||
P31 | pZS*1R | Venus | pSC101* | Amp | Yes | |||||
P32 | pZA32 | Venus | p15A | Cm | Yes | |||||
P33 | pZA31 | Venus | p15A | Cm | Yes | |||||
P34 | pZE12 | GFP | ColE1 | Amp | Yes | |||||
P35 | pLDR8 | Yes | ||||||||
P36 | [http://partsregistry.org/Part:BBa_I763004 BBa_I763004] | GFP + IPTG + LVA | Kan/Amp | |||||||
P37 | [http://partsregistry.org/wiki/index.php/Part:BBa_I722007 BBa_I722007] | Constructive expression LacI w/ pTetR promoter | Amp | |||||||
P38 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23114 BBa_J23114] | High constitutive promoter | Amp | |||||||
P39 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23113 BBa_J23113] | Low constitutive promoter | Amp | |||||||
P40 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0032 BBa_B0032] | Ribosome Binding Site | Amp | |||||||
P41 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0010 BBa_B0010] | Transcriptional Terminator | Amp | |||||||
P42 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0012 BBa_C0012] | LacI coding region | Amp | |||||||
P43 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0040] | TetR coding region | Amp | |||||||
P44 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0051 BBa_C0051] | cI lambda | Amp | |||||||
P45 | [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0240 BBa_E0240] | GFP only w/ RBS & terminator | Amp | |||||||
P46 | [http://partsregistry.org/Part:BBa_I51020 BBa_I51020] | Base Vector | Amp | |||||||
P47 | [http://partsregistry.org/Part:BBa_P1003 BBa_P1003] | Kan Resistance Cassette | Kan | |||||||
P48 | [http://partsregistry.org/Part:BBa_P1004 BBa_P1004] | Cm Resistance Cassette | Cm | |||||||
P49 | [http://partsregistry.org/Part:BBa_P1002 BBa_P1002] | Amp Resistance Cassette | Amp | |||||||
P50 | [http://partsregistry.org/Part:BBa_P1005 BBa_P1005] | Tet Resistance Cassette | Tet | |||||||
P51 | [http://partsregistry.org/Part:BBa_Q04121 BBa_Q04121] | LacI QPI w/ RBS & promoter | Kan | |||||||
P52 | [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510] | cI Lambda QPI | Kan | |||||||
P53 | [http://partsregistry.org/Part:BBa_P0440 BBa_P0440] | PoPS -> TetR | Amp | |||||||
P54 | [http://partsregistry.org/Part:BBa_P0412 BBa_P0412] | PoPS -> LacI | Amp | |||||||
P55 | [http://partsregistry.org/Part:BBa_P0455 BBa_P0455] | RBS.cI.LVA.Term | Amp/Cm |