Team:Harvard/Plasmids

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Vectors

Parts

Name Registry Name Description Origin Size Marker Transformed? Miniprepped? Culture in Glycerol Stock? Sequenced?
P1 J23113/I20259Low promoter GFP testerp15A pMR101-derived919Kanin S1 and TOP10Yes for both strains Yes for both strains.
P2 J23150/I20269Medium promoter GFP testerp15A pMR101-derived919Kanin TOP10, not S1
P3 J23151/I20270High promoter GFP testerp15A pMR101-derived919Kanin TOP10 and S1Yes for both strains.Yes for both strains.
P4 pACYC duetLow-Medium copy vectorp15a4008CmYesin S1 and TOP10
P5 pSB3K3Low-Medium copy vector (w/ death gene)p15a2750Kan
P6 BBa-E1010RFP only (w/ death gene)pMB1681Kan
P7 pSB1A2GFP onlypMB12079Amp
P8 BBa_J04450RFP with LacI promoterpMB11069Amp
P9 BBa_J04430GFP with LacI promoterpMB11083Amp
P10 BBa_I715038T7 Polymerase with LacI promoterpMB12878Amp
P11 BBa_M30109Light responsive system, dual regulationpUC19-derived pMB14333Amp/Cm
P12 pETDuet-1Low copy plasmidpBR322-derived ColE15420Amp
P13 pCDFDuet-1Low copy plasmidCloDF133781SmYesin S1
P14 pCOLADuet-1Low copy plasmidColA3719Kan
P15 BBa_I13522GFP with Tet promoterColE1937Amp
P16 BBa_R0040Tet repressible promoterpMB154Amp
P17 BBa_Q04400Inverter (TetR w/o promoter and Tet promoter)OriS, P1 lytic, F1902Kan
P18 BBa_R0051lambda promoter (cI regulated)pMB149Amp
P19 BBa_I763007RFP with lambda promoterpMB1918Amp
P20 BBa_J04431GFP (LVA tagged) with Lac promoterpMB11122Amp, Kan
P21 A1 (pACYC)pACYC (P4) w/ biobricks?????????Yesin S1 and TOP10
P22 BBa_R0082ompR controlled promoter for ompC (long)pMB1108Amp
P23 BBa_R0083ompR controlled promoter for ompC (short)pMB178Amp
P24 BBa_R0084ompR controlled promoter for ompFpMB1108Amp
P25 BBa_J58102Promoter activated by OmpR-P with the reporter GFPpUC19-derived pMB1991Amp
P26 BBa_J58103Mutated promoter activated by OmpR-P with the reporter GFPpUC19-derived pMB1961Amp
P27 pZS4int2repressor plasmidpSC101SmYes
P28 pZE21GFPColE1KanYes
P29 pZS*2RVenus (mutant YFP)pSC101*KanYes
P30 pZA32YFPp15ACmYes
P31 pZS*1RVenuspSC101*AmpYes
P32 pZA32Venusp15ACmYes
P33 pZA31Venusp15ACmYes
P34 pZE12GFPColE1AmpYes
P35 pLDR8Yes
P36 BBa_I763004GFP + IPTG + LVAKan/Amp
P37 BBa_I722007Constructive expression LacI w/ pTetR promoterAmp
P38 BBa_J23114High constitutive promoterAmp
P39 BBa_J23113Low constitutive promoterAmp
P40 BBa_B0032Ribosome Binding SiteAmp
P41 BBa_B0010Transcriptional TerminatorAmp
P42 BBa_C0012LacI coding regionAmp
P43 [1]TetR coding regionAmp
P44 BBa_C0051cI lambdaAmp
P45 BBa_E0240GFP only w/ RBS & terminatorAmp
P46 BBa_I51020Base VectorAmp
P47 BBa_P1003Kan Resistance CassetteKan
P48 BBa_P1004Cm Resistance CassetteCm
P49 BBa_P1002Amp Resistance CassetteAmp
P50 BBa_P1005Tet Resistance CassetteTet
P51 BBa_Q04121LacI QPI w/ RBS & promoterKan
P52 BBa_Q04510cI Lambda QPIKan
P53 BBa_P0440PoPS -> TetRAmp
P54 BBa_P0412PoPS -> LacIAmp
P55 BBa_P0455RBS.cI.LVA.TermAmp/Cm
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