"
Page
Discussion
View source
History
teams
Log in
Team:Harvard/Recipes
From 2008.igem.org
Revision as of 22:25, 28 October 2008 by
Joyy
(
Talk
|
contribs
)
(
diff
)
← Older revision
|
Latest revision
(
diff
) |
Newer revision →
(
diff
)
Home
Team
Project
Overview
Organism
Hardware >>
Fuel Cell Development
Experimental Procedures
References
Parts Made
Overview
lacI System
Temperature
sensitive cI
Other
Notebook
Daily Notebook
General Protocols
Recipes
Plasmids
Recipes
Contents
1
Recipes
1.1
Orianna's growth media
1.2
LML medium (for anaerobic Shewanella growth)
1.3
LML agar (for Shewanella reduction test)
1.4
Chamber media
1.5
Phosphate buffer
Orianna's growth media
50 mM K-PIPES
18.925 g/L
7.5 mM NaOH
7.5 mL/L
28 mM NH
4
Cl
1.5 g/L
1.3 mM KCl
433 uL/L
4.3 mM NaH
2
PO
4
0.59 g/L
100 mM NaCl
5.84 g/L
18 mM Lactate
1.62 g/L
10 mL Vitamin solution
10 mL Amino acid solution
10 mL Trace mineral solution
LML medium (for anaerobic Shewanella growth)
0.02% yeast extract
0.01% peptone
20 mM lactate
10 mM HEPES [pH 7.4]
LML agar (for Shewanella reduction test)
LML medium
0.8% agar
2 mM MnO2- Mn(IV) oxide (turns from brown to clear upon reduction to Mn(II))
(LML recipes from
Beliaev and Saffarini 1998
)
Chamber media
5.844 g/L 100mM NaCl
15.1185 g/L 50mM PIPES (hydrogen)
7.0 pH
Phosphate buffer
2.918 g/L Monosodium phosphate, monohydrate
4.095 g/L Disodium phosphate, anhydrous
5.844 g/L 100mM NaCl
7.0 pH