Team:Hawaii/Birds Eye View

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Oahu

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Our team aims to create the framework and tools for expanding the scope of BioBrick synthetic biology to organisms other than E. coli. Creation of a mobilizable broad-host-range plasmid enables the transfer of modularized and abstracted genetic components to act in wide range of organisms that share a similar pool of intermediate metabolites. A broad-host-range mobilizable vector would serve as a channel to "open source" the rich diversity of genetic information between organisms in a laboratory setting. We envision a wide diversity of future broad-host-range BioBrick parts, created and transported, with this or similar BioBrick vectors; each designated to be compatible with specific organism(s). These modules can also be combinatorially transformed or combined into organisms as larger devices to produce desired products. more...

Team Team

Logistics

Sponsors

Dr. Gary Ostrander, Vice Chancellor for Research & Graduate Education

Dr. Andrew Hashimoto, Dean, College of Tropical Agriculture and Human Resources

Dr. Gernot Presting, Assistant Professor, MBBE

Projects Projects

Part A: Mobilizable Broad-Host-Range Plasmid Part B: Cyanobacterial protein secretion system

Experiments (t)

Notebook (t)

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Events Events

Milestones

  • Culturing Protocol Optimization: CO2 is bottle neck, need incubator retrofitted (KS)
  • Express GFP constructs in Synechocystis

Meetings (t)

Mon. 12-1 or Fri. Afternoon (as needed basis)

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Resources Resources

Parts

Design

Help

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Protocols (t)


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