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Team:Hawaii/Experiment
From 2008.igem.org
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
In Progress
- Ligation of pRL1383a Parts
- Construction of BioBrick intermediates
- Construction of Broad-Host-Range Expression Vector
- Construction of Omega Interposon BioBrick
- Antibiotic test for aadA construct
- Conjugation Test: Comparing oriT with oriTr
Not Started
Completed
- PCR Amplification of pRL1383a
- PCC6803 cell density test
- PCC6803 Cryostock Thaw Test
- Effect of CO2 on Growth Experiment
- Effect of Water Motion on Clumping of Liquid Cultures
- Make Competent E. Coli
- Test Competent E. Coli
- Initial E. Coli Transformation
- Initial BioBrick Plasmid Extraction From Filter Paper
- Biobricks extraction/transformation competency test
- Initial PCC6803 Transformation
- Tri-parental conjugation between E. coli and PCC6803
- Mini Plasmid Prep
- Large-Scale Preparation of Plasmid
- Initial Synthetic Oligonucleotide Assembly
- Conversion of GFP into a fusion brick and pRL1383a into a Biobrick vector
- Antibiotic test for BB-pRL1383a
