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Team:Hawaii/Notebook/2008-10-16
From 2008.igem.org
Revision as of 03:25, 19 October 2008 by MargaretRuzicka (Talk | contribs)
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colonies |
|---|---|
| nir + rbs+GFP+tt in BBpRL1383a-1 | 2 |
| plac + rbs+GFP+tt in BBpRL1383a-1 | 0 |
| J33207 in pRL1383a (all plates) | lawn |
- Colony PCR of transformants
- None of the colonies tested positive for their desired inserts
Transformation
- Margaret
| Construct | Colonies |
|---|---|
| lac promoter/rbs(34)+ rep + pSB1A2 | 0 |
| lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) +pSB1A3 | 113 |
| lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (A) | 141 |
| lac promoter/rbs(30)rep/oriV (6) + lac promoter/rbs(34)aada/oriT (B) +pSB1A3 | 150 |
| oriV (6) + oriT + pSB1A7 | 200 |
| pSB1A7 self (did not de-phosphorylate) | 50 |
| DH5-a with RP4 | 3 |
| DB3.1 with RP4 | 0 |
| (+) pUC18 | lawn |
| (-) no plasmid | 0 |
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
