Team:Hawaii/Notebook/2008-10-27

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Things we did today

Wetlab work

Triparental conjugation

Grace
Liquid and solid media cultures of triparental conjugation of Synechocystis. No growth was observed in any of the cultures.
  • Checked on triparental conjugation cultures from 10/13. No growth was observed in any of the cultures.
  • E. coli were grown at too low antibiotic concentration (sp2.5). Cells probably lost plasmid during growth, before conjugation. Will repeat triparental conjugation on Wednesday.

Plasmid prep of BBpRL1383a

Grace
  • Used Qiaprep Spin Miniprep Kit to prep the rest of the BBpRL1383a liquid cultures.

Cryostocked BBpRL1383a

Grace
  • Cryostocked 10/22 culture of DH5α w/ BBpRL1383a

Restriction digest

Grace
  • 6 hour XbaI/SpeI digest (for pretty picture):
  • nir, nir+rbs, slr, pilA, slr+GFPf, GFPf
  • Overnight HindIII/BamHI digest:
  • J33207

Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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