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Team:Hawaii/Notebook/2008-10-28
From 2008.igem.org
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= Things we did today = | = Things we did today = | ||
== Wetlab work == | == Wetlab work == | ||
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===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]=== | ===[[Team:Hawaii/Antibiotic_test_for_BB-pRL1383a |Antibiotic test]]=== | ||
Revision as of 22:32, 29 October 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Antibiotic test
- Grace
File:102808j33207.jpg
EtBr stained 2% agarose gel ran at 60V for 2 hours. Twenty microliters of HindIII/BamHI digested J33207 were loaded.
- Ran HindIII/BamHI digested J33207 on gel
- Extracted from gel
- Ligated with HindIII/BamHI digested pRL1383a
- Used 5 μl ligation reaction to transform 100 μl DH5α MCR cells (from Doug in SC lab)
- Also transformed 25 μl DH5α MCR with 2 μl BB-1 1025 and 1 μl J33207 each.
Drylab Work
Project page
- Grace
- Wrote up Materials, Methods and Results for Project Part B on team project page.
Sequence analysis
- Grace
- Same G->C transversion in CAP binding site for BBpRL-1 sequence.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
